Atlas ELISA Kits
Simple 1-step intact EV quantification
The Atlas EV ELISA detects and quantifies the tetraspanins CD9, CD63 and CD81 simultaneously on intact vesicles, run directly from biofluids or SEC fractions, using just 50 µL of sample.
- One-step assay against CD9, CD63 and CD81
- Just 50 µL: no purification or concentration needed
- Absorbance and chemiluminescence (Lumi) formats
- 12 × 8-well strips: run a few wells or a full plate
The Atlas EV ELISA provides a robust, high-throughput, plate-based solution for quantifying extracellular vesicles (EVs) and targeted surface markers directly from biofluids or purified samples. Unlike particle-based methods such as NTA or flow cytometry, Atlas delivers specific, reproducible results with minimal sample prep, 15 minutes hands-on time and only 50 µL input.
Atlas ELISAs from Everest Biolabs are available in two formats:
- Absorbance ELISA - Simple and reliable EV yield and purity assessment using standard plate readers.
- Chemiluminescence (Lumi) ELISA - High sensitivity, wide dynamic range, and customizable capture (Homebrew options) for profiling disease-relevant markers.
Applications include optimizing SEC fractionation, comparing EV yield vs purity, validating against NTA/flow cytometry, and detecting low-abundance, tissue-specific markers with the Lumi Homebrew kit.





