Thermo Scientific™ Vanquish™ Flex Quaternary UHPLC System

In this application note, Thermo Fisher Scientific proposes an HPLC-based approach to monitor the characteristics of PS 80 samples that provide operational simplicity while generating a high degree of information.

Mass spectrometry is a popular choice of technology in clinical research for the quantitation of immunosuppressant drugs in whole blood samples. A sensitive and robust instrument is required for reliable quantitation workflows. In this study, the robustness of the TSQ Quantis triple quadrupole mass spectrometer for analysis of immunosuppressant drugs in whole blood samples in demonstrated.

In this application note, an analytical method for clinical research for the quantification of 23 antidepressants in human plasma or serum is reported. The analysis includes:

atomoxetine, bupropion, citalopram, desmethylfluoxetine, desmethylmirtazapine, desmethylsertraline, dosulepin, duloxetine, threo-dihydrobupropion, fluoxetine, fluvoxamine, mianserin, milnacipran, mirtazapine, moclobemid, hydroxybupropion, opipramol, paroxetine, reboxetine, sertraline, tranylcypromine, trazodone, and venlafaxine.

This application note describes a quantitative and sensitive method for the analysis of estradiol and testosterone in plasma using LC-MS/MS.

In this application note, a clinical research analytical method for the quantification of 35 benzodiazepines in human plasma or serum is reported. The panel includes :

3-hydroxybromazepam, 7-aminoclonazepam, 7-aminoflunitrazepam, 7-aminonitrazepam, α-OH-alprazolam, α-OH-midazolam, α-OH-triazolam, alprazolam, bromazepam, chlordiazepoxide, clobazam, clonazepam, demoxepam, desalkylflurazepam, desmethylflunitrazepam, diazepam, estazolam, flunitrazepam, flurazepam, lorazepam, lormetazepam, medazepam, midazolam, nitrazepam, norclobazam, nordiazepam, oxazepam, prazepam, temazepam, tetrazepam, trazodone, triazolam, zaleplon, zolpidem, and zopiclone.

In this application note, the MAbPac-RP column was operated with the Vanquish Flex UHPLC system for the reversed-phase chromatography of several intact therapeutic antibodies. To assess the suitability for stability studies, the chromatograms of a reference and a stressed mAb were compared.

This application shows an easy deamidation monitoring using the Vanquish Flex UHPLC system with UV detection. Therapeutic proteins have a major role in today’s medical treatment for a wide array of diseases. The diversity and efficacy of biotherapeutics makes them ideally suited for the treatment of various diseases such as rheumatoid arthritis, multiple sclerosis, and several types of cancer. Due to the complexity of biotherapeutics, they are prone to post-translational modifications that have to be characterized. One of the most common non-enzymatic post-translational modifications in therapeutic proteins is the deamidation of asparagine residues. It has been reported that deamidation can lead to allergic reactions as well as a reduction of the half-life, pharmacological dynamic, stability, or even a loss of the biological function. Thus it is crucial to characterize and control deamidation products in biopharma processes to ensure a high quality product.

In this product spotlight, learn more about the Vanquish Flex UHPLC System. No matter what questions need to be answered, all chromatographers rely on the reproducibility of their UHPLC system. Superior retention time and peak area precision is of paramount importance for data confidence, as well as the instrument’s ability to produce consistent results every single time. The Thermo Scientific™ Vanquish™ Flex UHPLC system has been designed to fulfill these needs.

Peptide mapping is one of several routine methods to characterize biopharmaceutical proteins. For research environments, this technique, if combined with mass spectrometry (MS), is utilized for the characterization and confirmation of the primary sequence of monoclonal antibodies. In addition, peptide mapping can help to identify, localize, and quantitate post-translational modifications (PTMs). Peptide mapping methods are often developed and evaluated with combined UV and MS detection, to simplify the transfer to routine environments where UV detection is used alone. In high-throughput workflows, peptide mapping experiments are performed for antibody identity confirmation, PTM characterization, and stability studies. In this work, the separation of peptides obtained from a monoclonal antibody digest is demonstrated with a LC-UV-MS setup.

Peptide mapping is one of the routine methods for biotherapeutics characterization. This technique, combined with mass spectrometry, is utilized in research environments for the determination of the primary sequence of an antibody or the identification of post-translational modifications. UV is often used as a unique detection tool, for instance in stability studies. The data interpretation of these experiments is based on retention times as qualitative and peak area as quantitative information. In this application note, the separation of peptides obtained from a bovine serum albumin (BSA) digest is demonstrated. The retention time and peak area precisions are evaluated for repeated injections.

Discover how Dr. Erika Castrignanò, Principal Analyst at King's College London's Drug Control Centre, employs liquid chromatography and mass spectrometry for sports testing. Explore the principles of wastewater-based epidemiology and its impact on combating doping in elite sports, as well as understanding drug consumption in wider communities.

Professor. Kim Wolff, Director of King's Forensics and Head of the Drug Control Centre at King's College London, specializes in forensic toxicology and anti-doping science. Wolff discusses the overlap between the two fields, emphasizing the need for sensitive methods to detect drugs at major events. Find out how innovations like dried blood spot testing and a new instrument from Thermo Fisher Scientific, helps Wolff’s team differentiate between synthetic and natural steroids used by athletes.

Join Christiaan Barlett, Lab Operations Manager at King's College London's Drug Control Centre, as he discusses athlete biological passports, an innovative system for the detection of blood, steroid, and growth hormone doping, ensuring fair competition in elite-level sporting events like the Olympics. During the Paris 2024 Olympic games, the turnaround time for passport testing changes from 20 days to an astonishing 24 hours, so that cheats can be detected quickly. Bartlett explains how this is possible with the highest possible accuracy.

Watch this video to find out how to swap out your current instrumentation with ease thanks to the Lab-Forward Trade-In Program.

Combining the separation power of the Vanquish series with boundary-pushing flexibility, Evert-Jan Sneekes, HPLC Strategic Marketing Manager at Thermo Fisher Scientific, explains how the Vanquish™ Flex system gives you better separations and more results. The Vanquish™ Flex system integrates modularity and state-of-the-art quaternary solvent blending to extend the capabilities of the Vanquish platform, and its biocompatibility makes it ideal for biopharmaceutical applications. From peptide mapping to aggregate analysis, have confidence in your results with the Vanquish™ Flex.

Thermo Scientific Tox Explorer Collection provides instruments, columns and consumables with reliable and validated methods for reproducible results

Collect our three guides for robust and reliable technologies to enable scientists in forensic and clinical laboratories

Supra Research CEO Dr. Rob O’Brien gives insight on its analytical processes, current challenges and a breakthrough method for cannabis testing in chocolate for the Canadian market

Join our free upcoming webinar for expert insight from chromatography experts

Thermo Scientific Q Exactive BioPharma enables three powerful new protein characterization workflows on one instrument Researchers performing biopharmaceutical characterization can now benefit from a single system

Thermo Scientific Vanquish Flex Binary UHPLC system boosts high-speed applications and provides exceptional LC and LC-MS performance

Hear how the biocompatible UHPLC system will give you better results

Select Science reports on the latest technologies from HPLC 2015

SelectScience attended this year's HPLC show to bring you the latest advances in high performance liquid chromatography.