Raptor™ Biphenyl LC Column

Achieving reliable separations in complex analyses has long challenged chromatographers, especially when working with early-eluting or closely related compounds. Traditional phases often fall short, requiring intricate mobile phases that complicate MS detection.

Learn how enhanced selectivity and robust performance can simplify method development, accelerate separations, and support confident results, even in the most demanding LC-MS workflows.

In this poster, Restek outlines a chromatographic method that efficiently separated 12 mycotoxins used in the analysis of four different commercially avaliable matrices. Here, Restek compares stable isotope dilution assays (SIDAs) with matric-matched calibration methods by LC-MS/MS.

Vitamin K₁ and K₂ analysis is typically complex and time-consuming because these lipophilic vitamins occur at low levels and are subject to matrix interference, particularly from phospholipids. The new method established here provides fast, accurate, and precise vitamin K analysis in plasma, using a simple phospholipid removal procedure and a sensitive, 4-minute LC-MS/MS analysis on a Raptor Biphenyl column, making it suitable for both research and high-throughput testing.

Clinical diagnosis of pheochromocytoma and paraganglioma is often based on the analysis of catecholamines (epinephrine, norepinephrine, dopamine) and metanephrines (metanephrine, normetanephrine, 3-methoxytyramine) in urine. Analysis of these polar compounds using reversed-phase LC can be difficult due to limited chromatographic retention, which results in poor separation of the analytes from closely eluting matrix interferences. This method overcomes these problems by combining a simple solid phase extraction procedure with the consistent and accurate chromatographic performance of a Raptor Biphenyl column.

Bisphenol A (BPA) is a high production volume industrial chemical used to make polycarbonate plastics and epoxy resins that are used in many industries. BPA is released into the environment through industrial effluents and can also leach from packaging materials into food and beverage products. Although BPA concentrations are often low, repeated daily exposure can result in high cumulative doses. Interest in BPA analysis is growing as bisphenol A is an endocrine disruptor that can mimic estrogen, thus disrupting normal hormone levels and causing a significant impact on health. It is also potentially linked to obesity, diabetes,
ADHD, and other health conditions. BPA HPLC analysis can be done using a C18 LC column, but analysis times can be long and peak shape often is not ideal.

In this technical note, Restek demonstrates a simple dilute-and-shoot method for fast analysis of fentanyl and related compounds in human urine by LC-MS/MS using a Raptor Biphenyl column.

Accurate analysis of aldehydes and ketones in air samples is an important part of monitoring air pollution and protecting human health around the world. These airborne carbonyls from industrial and vehicle emissions are precursors to ozone in smog and are associated with respiratory and pulmonary problems. The following application note details an improved methodology for this analysis.

The analysis of synthetic cannabinoids and their metabolites can be a difficult and challenging task. Keeping up with the ever-growing list of synthetic cannabinoids that illicit drug makers produce further complicates the analysis. This application note shows how the retention and selectivity of the Raptor Biphenyl column enable new drugs to be added to an existing LC-MS/MS method.

In this partial validation study, the performance of a fast dilute-and-shoot LC-MS/MS method is demonstrated using a Raptor™ Biphenyl column for the simultaneous analysis of 5-hydroxyindoleacetic acid (5-HIAA), vanillylmandelic acid (VMA), and homovanillic acid (HVA) in human urine. Excellent results were obtained for linearity, accuracy, and precision. With this method, these serotonin and catecholamine metabolites can be measured in human urine with a 5-minute total analysis time. The analytical method is applicable to clinical labs that require a combination of fast analysis times and low-cost sample preparation procedures for 5-HIAA, VMA, and HVA using LC-MS/MS analysis.

A rapid, accurate, and reproducible method was developed for high-throughput testing of nicotine, cotinine, trans-3'-hydroxycotinine, nornicotine, norcotinine, and anabasine in urine. Data show that a fast and highly efficient analysis of these basic compounds can be achieved with the Raptor™ Biphenyl column using standard low-pH, reversed-phase LC-MS mobile phases that are compatible with a variety of LC-MS instrumentation.

Tuesday, November 18, at 16:00 GMT | 17:00 CET | 11:00 EST | 8:00 PST

Developing a new HPLC method often follows a familiar routine in many laboratories: select a C18 column, run a few test injections, and hope for sufficient retention and separation. While C18 remains a widely trusted and globally adopted stationary phase, this webinar introduces a fresh perspective on what’s often considered a 'generic' choice.

Join Application Scientist, Samantha Herbick as she explores the retention profiles of various C18 columns, highlighting that not all C18 phases are created equal, and examines the structural modifications permitted within the USP L1 classification.

She will also introduce biphenyl phase and compare it to C18, showcasing how its unique ligand chemistry offers alternative retention mechanisms that can reshape your approach to method development.

Key learning objectives:

• Explore an overview of the retention mechanisms at play with various C18 column types
• Discover how biphenyl columns retain, how they differ from C18, and how you can benefit from these differences
• Uncover the best practices for successful utilization of biphenyl columns in LC methods
• Hear examples of biphenyl chromatography in applied solutions, with contrast against C18 in the same application

Who should attend?

• Analysts, method development specialists, research chemists, lab managers, and anyone interested in LC-MS.

Certificate of attendance
If you attend the live webinar, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

If you view the on-demand webinar, you can request a certificate of attendance by emailing editor@selectscience.net.

In this video, Restek describes how to properly condition your LC column. Clean, stable baselines are critical to good liquid chromatography, and properly conditioning your LC column is a helpful way to ensure a good baseline. You’ll need to flush out the storage solvent and equilibrate the column to your solvent mixture, and you may need to use an intermediate solvent to ensure complete mixing and flushing.

In this video, Restek discusses LC column cleaning and regeneration. Watch to learn the steps you can take to keep your column clean and help restore some performance to columns and extend their lifetime.

Tailing peaks can be a problem when doing liquid chromatography (LC), and secondary silanol interactions are one of the causes. Here, using the example of basic drugs, Restek explains what causes them and cover a few steps that can be taken to minimize the effect of these secondary silanols on peak shape when troubleshooting.

A growing need is emerging for testing to detect the presence of mycotoxins in both raw plant materials and finished cannabis products in order to protect consumer health and safety. The analysis of mycotoxins in cannabis oils is particularly challenging because the sample lipids can contribute isobaric matrix interferences in addition to general ion suppression, which can reduce accuracy especially at low levels. This video presents a simple pass-through SPE sample cleanup method applied to lipid-rich CBD oils. The SPE sample cleanup was paired with a rapid LC-MS/MS analysis utilizing a Raptor Biphenyl column.

This video introduces the chromatography technology from Restek, showing how it can be applied to a variety of fields and improve lab performance.

A breakthrough for rapid screening, the technology aims to bring a new level of efficiency and speed

Watch this on-demand webinar to learn about recent advances in chromatography technology and how to apply them to food commodity testing workflows

Paul Connolly from Restek shares the secrets to success for its most popular LC stationary phase, in this SelectScience interview

As the possibility of federal legalization looms in the U.S., hear how Restek is approaching cannabis for 2020 and working with its customers to advance the field of cannabis testing as a whole

Choose the right mobile phase chemistries to speed up analysis while maintaining good peak resolution in challenging matrices

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