MALDI SYNAPT G2-Si High Definition Mass Spectrometry

Ion mobility separates ions according to their size and shape, and reports the separation as either the drift time or the collision cross-section (CCS). This application note explores the ion mobility utility of the Waters Vion MS and SYNAPT platforms for CSS analysis of pharmaceuticals.

This application note describes the use of AccQ-Tag™ Ultra reagent, combined with UPLC-ESI-MS/MS, for the analysis of amino acids and biogenic amines in human plasma or serum, via a high-throughput, sensitive, and specific method.

This is a compilation of application notes from Waters Corporation focused on mass spectrometry imaging and ambient ionization mass spectrometry applied to metabolomics and lipidomics.

This application note demonstrates that by optimizing DESI conditions, a wealth of molecular information can be accessed from a single tissue section, without the need to substantially alter the analysis conditions. Mass spec imaging of tissue sections was accomplished by using a SYNAPT® G2-Si HDMS Mass Spectrometer equipped with a 2D-DESI source. Data was generated and analyzed using HDI Software v1.3.

This application note form Water Corporation compares two imaging methods for tissues, DESI and MALDI to see if the tissue is modified in any way. The tissue used was frozen colorectal tumor mounted on glass sides.

Matrix assisted laser desorption ionization (MALDI) mass spectrometry imaging (MSI) coupled with ion mobility separation has demonstrated significant utility over the last decade for the separation of matrix ion species from endogenous ion species in the gas phase. Desorption electrospray ionization (DESI) has been gathering momentum as a complementary MSI technique to the more traditional SIMS and MALDI-MS approached. This poster compares and contrasts DESI imaging with MALDI imaging on the same ion mobility enabled mass spectrometer.

This poster describes a fast and robust microfluidics platform for the analysis of lipidomics that increased the sensitivity whilst considerably reducing the solvent consumption. This novel platform integrates the UPLC separation into the source of the mass spectrometer.

This application note explains the fractionation of Host-cell proteins (HCPs) away from biotherapeutic protein using an off-line preparative electrophoresis device, allowing for sub-ppm level HCP detection. HCPs are proteins produced by the host cells during recombinant protein drug production and can contaminate the therapeutic drug protein even at very low levels. Detection of HCPs was improved through fractionating both the intact and reduced monoclonal antibody.

This application note demonstrates how increased confidence in the identification of extractable or leachable compounds can be provided by the inclusion of collisional cross section (CCS) values, acquired using ion mobility-mass spectrometry. The CCS depends on an ion's size, shape and charge, and is a key physicochemical property of compounds.

This application note addresses the increased concern regarding the authenticity of basmati rice by analysing volatile compounds of interest extracted via SPME/headspace. After GC separation, detection was performed using APCI ionization on a SYNAPT G2-Si.

In this video, discover how the spatial distribution of oncological therapies can be characterized using matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI MSI) technology. Using the mimetic model protocol and the MALDI SYNAPT G2-Si high definition mass spectrometer from Waters Corporation, Dr. Bindesh Shrestha demonstrates the semi-quantification of the chemotherapy drug, doxorubicin.

This interview was filmed as part of our coverage of AACR 2019 - see other video highlights here >>

Hear how Waters technology is being used to analyze the heaviest of crude oils. Ion mobility provides data and analytics that no other technique can, due to the high boiling points of the crude oil fractions, so the Synapt G2-Si is ideal.

Watch this video to discover more about how the Waters SYNAPT series has made dynamic virus analysis under a range of temperatures possible. Dr Ganesh Anand, of the National University of Singapore, and a Waters Center of Innovation Program honoree, describes how he uses hydrogen deuterium exchange mass spectrometry to structurally analyze biological molecules.

Q-TOF instruments from Waters have been used to revolutionize the characterization of proteins at Ohio State University. Watch this video to learn how the technology has been used to study protein complexes, with greater ease than using traditional NMR methods.

As the use of sports supplements by athletes continues to rise, hear how Inmetro uses mass spectrometry and proteomics analysis to test and regulate the content of these performance-enhancing products.

IMMray™ PanCan-d blood-based test for early detection of pancreatic cancer now set for Q3 2020 launch

We look back at the 2019 American Association for Cancer Research (AACR) annual meeting and the technology set to propel cancer research

Don’t miss April’s highlights thanks to our regular monthly round-up of all the most popular stories and resources in the clinical science community

Learn how innovative mass spectrometry techniques are enabling scientists to explore the molecular make-up of tumors — opening the way to better drug efficacy and new treatment strategies

Read this exclusive interview with Louise Kenny, one of the founding directors of the INFANT Centre, to discover how they are using mass spectrometry to research preeclampsia and pre-term birth

Powerful software allows for full integration of multimodal mass spectrometric imaging experiments in a single user interface

Discover the latest products and methods for the imaging of biological samples

Studying proteomics and metabolomics with TOF mass spectrometers from Waters

New Products, Videos and Application Notes