FastDNA SPIN Kit for Soil™

Infants born prematurely, particularly extremely low birth weight infants (ELBW), have altered gut microbial communities. There is a requirement to optimally characterize microbial profiles in this at-risk cohort, via standardization of methods, particularly for studying the influence of microbiota therapies (e.g. probiotic supplementation) on community profiles and health outcomes. Profiling of faecal samples using the 16S rRNA gene is a cost-efficient method for large-scale clinical studies to gain insights into the gut microbiota and additionally allows characterization of cohorts where sample quantities are compromised. To this end, the bacterial DNA extraction protocol from ELBW infant faeces was optimized by testing three different DNA extraction methods.

The challenge with extractions from soil is isolating DNA or RNA without contamination by humic acids or other PCR inhibitors. Volcanic ash soil, such as andisol, is known to be very difficult because this soil has a complex matrix. In this application note, learn how to improve DNA extraction.

In this application note, learn how MP Biomedical assess microbial communities present in wastewater samples.

In this application note, from MP Biomedical, learn how to perform the FDA’s method for detection of C. Cayetanensis in fresh produce items.

In this application note, learn how a combination of the FastDNA SPIN Kit for Soil and the FastPrep-24 instrument from MP Biomedicals provide a high quality microbial genomic DNA from soil.

Download this method to learn how the FastPrep-24™ homogenizer can be used used in combination with the FastDNA™ Spin Kit for Soil and is shown to be an effective method for the lysis of C. cayetanensis oocysts from infected food matrices and isolation of their DNA. The extracted DNA was used successfully in real-time PCR assays that were able to detect as few as 5 oocysts in 25 g of food samples.

In this application note, MP Biomedicals illustrates how its FastDNA Kit, FastDNA Spin Kit, and FastRNA Pro Green Kit make extracting enough usable DNA or RNA from plant samples quick and easy. This example explains how you can use these solutions to extract RNA from cassava roots in only 60 seconds, resulting in effective and efficient sample preparation for PCR analysis.

The primary challenge with wastewater is that it contains many PCR inhibitors and contaminants. Extracting a high-quality DNA or RNA for PCR analysis and other downstream applications is made easily and quickly possible with the FastDNA Spin Kit for Soil or the FastRNA Pro Soil Kits.

This application note demonstrates how to quickly and easily extract usable DNA or RNA from plants for PCR analysis and other downstream applications.

The challenge with extraction from soil is isolating DNA or RNA without contamination by humic acids or other PCR inhibitors. The FastDNA Spin Kit for Soil and FastRNA Pro Soil Kits used in combination with the FastPrep instrument will help overcome any difficulties with complete lysis of all soil organisms including historically difficult sources such as eubacterial spores and endospores, gram positive bacteria, yeast, algae, nematodes and fungi, and isolation of pure DNA and RNA.

To explore the natural microbial community of any ecosystem by high-resolution molecular approaches, it is extremely important to develop sensitive and reproducible nucleic acid extraction methods that facilitate isolation of microbial DNA and RNA of sufficient purity and quantity.

Proper lysis of microbial cells without damaging their genomes is the first major challenge. Then, as a second step, isolation and extraction must yield high quality nucleic acid for subsequent metagenomic studies.

MP Biomedicals supplies a complete solution from sample grinding, cell lysis to nucleic acid extraction and purification.

In order to offer a ready-to-use, flexible and reliable solution for nucleic acid isolation, MP Biomedicals has developed a portfolio of FastPrep bead beating instruments together with a broad panel of Lysing Matrix tubes to grind and lyse thoroughly any sample type and built up a comprehensive range of organic-based, spin column-based as well as magnetic beads-based extraction kits for the isolation of high-quality DNA and RNA.

Key learning objectives

• Learn how to obtain thorough homogenization and lysis of any microorganism from samples used in microbiome research
• Discover how the FastDNA™ SPIN Kit for Soil from MP Biomedicals has been found to be one of the most suitable kits available for DNA extraction and microbiome sequencing
• Be informed of the new DNA isolation kits developed by MP Biomedicals to generate high yields and achieve rapid isolation of genomic DNA from environmental samples and water samples
• Discover the MPure-32™ Automatic Nucleic Acid Purification instrument designed for high-throughput applications and combined kits specially developed for DNA purification from various samples including feces and environmental samples

Who should attend?

• Scientists involved in metagenomic analysis of human and animal microbiome samples (biomedical research & veterinary research)
• Researchers working in the environmental and agricultural research field (with soil, sediment, water, sludge, compost, rhizosphere, wood, any other plant specimens as well as food samples)
• Scientists investigating bioenergy production processes

Certificate of attendance

All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

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