ResourceLife Sciences
Extraction of DNA/RNA from Plant Samples for PCR Analysis
12 Dec 2018This application note demonstrates how to quickly and easily extract usable DNA or RNA from plants for PCR analysis and other downstream applications.
This application note demonstrates how to quickly and easily extract usable DNA or RNA from plants for PCR analysis and other downstream applications.
Isolate Pure DNA from Cells Present in Soil or Other Environmental Samples! Thorough lysis in seconds of any microorganism present in environmental samples. Ready-to-use DNA for quantitative and qualitative characterization of microbial soil communities. Excellent reproducibility for an optimum assay-to-assay consistency. Total removal of humic acids and PCR inhibitors for a successful investigation of microbial diversity. The FastDNA™ SPIN Kit for Soil is designed to efficiently isolate bacterial, fungal, plant and animal genomic DNA from soil and environmental samples. Up to 500 mg soil are processed by the FastPrep®-24 5G or and any bead beating homogenizers with the Lysing Matrix E tubes designed to efficiently lyse all microorganisms including difficult sources such as eubacterial spores and endospores, gram positive bacteria and yeast. The released DNA is purified by a silica-based spin filter method and is suitable for PCR and other downstream applications.
Delivering the most effective grinding for all kind of sample in 40 seconds or less.
The FastPrep-24™ 5G Instrument is quite simply the most advanced sample preparation system ever! The only high-speed benchtop homogenizer featuring microprocessor-controlled, Hi-Def Touch Screen Programming makes the 5G the ultimate in ease, speed and performance for sample preparation applications where it is necessary to grind, lyse, or homogenize difficult and routine samples, allowing easy and reproducible isolation of stable RNA, active proteins and full-length genomic DNA. Applications include but are not limited to: biological tissues, fluids and excretions cell cultures environmental sample other inorganic solid matrices. The FastPrep-24™ 5G Instrument uses a unique, optimized motion to disrupt cells through the multidirectional, simultaneous beating of specialized Lysing Matrix beads on the sample material. Samples and buffers are added to Lysing Matrix Tubes containing specialized Lysing Matrix beads, supplied ready-to-use, certified nuclease-free and in a variety of sample type specific compositions. The sample tubes remain securely sealed during the processing and the single use design eliminates cross-contamination. Program parameters are easily set using the touch screen user interface, or choose from many Recommended Programs stored on the 5G's onboard computer. With a simple touch of the "run" button, in less than a minute your samples are completely lysed, super fast, every time! The highest power settings available ensure that DNA, RNA and proteins, even from difficult samples, are quickly liberated into the protective buffer, thus preserving their biological function. FastPrep-24™ 5G Exclusive Features Powerful - Highest speed range available, ideal for a wide range of applications and protects your sample Optimized - Cycle and pause programmable for true walk-away operation Convenient - >70 Recommended Programs for easy optimizations Intuitive - Interactive user-friendly interface and touchscreen Flexible - Optional sample holders allow processing from 2 - 50 mL tubes, under ambient or cryogenic conditions Smart - Integrated USB Port allows for software updates to be installed, and run history data to be downloaded to a laptop Safe - Electromechanical dome latch automatically secures processing chamber, with a simple finger touch Complete - Lysing Matrix Tubes and Purification Kits from One Source
Rapid Method of Isolating Pure Genomic DNA from a Wide Variety of Sources! Isolate PCR-ready DNA from a variety of sample types No hazardous organic reagents are required The FastDNA™ SPIN Kit quickly and efficiently isolates genomic DNA from almost any sample (plant and animal tissues, cultured cells, bacteria, yeast, fungi, insects, etc). Up to 200 mg of tissue or cells are processed by the FastPrep-24™ 5G, FastPrep FP120™, FastPrep-96™ or SuperFastPrep-1™ with the Lysing Matrix A tubes. The kit includes 3 different chaotropic buffers for the homogenization of a wide variety of sample types and the released DNA is purified by a silica-based spin filter method. Purified DNA is ready for enzyme digestion, electrophoresis, PCR and any other desired application.
