Real-Time PCR (qPCR) Mastermixes and Kits Products & Reviews

Especially designed for an easy and fast detection of mycoplasma in cell-based therapeutics like autologous chondrocyte transplants (ATMPs –advanced therapy medical products). Due to the short shelf life of these therapeutics, a fast result is needed. The PCR-technology makes it possible to reduce the time-to-result to only 3 hours.

Brilliant III Ultra-Fast SYBR® QPCR and QRT-PCR Master Mixes deliver superior specificity by utilizing a novel faster-activating hot start method to minimize the formation of primer-dimers and other off-target reactions. 

Rapidly detect Salmonella species in food and environmental samples: The Thermo Scientific™ SureTect™ Salmonella Species PCR Assay offers a rapid real-time PCR method for the enrichment, detection and confirmation of Salmonella species in food, animal feed products, including large sample sizes, and primary production samples (PPS).

The GoTaq®Real-Time PCR Systems are ready-to-use 2X master mixes optimized for quantitative PCR in dye (using BRYT(R) Green Dye) or hydrolysis probe detection format.   The 1-Step and 2-Step RT-qPCR Master Mixes contain GoScript™ Reverse Transcriptase for efficient first-strand cDNA synthesis.     GoTaq Real-Time PCR Systems Features & Benefits: Higher fluorescence, often resulting in earlier Cqs Direct su…

TaqMan® Gene Expression Assays are a comprehensive collection of over 1,000,000 probe and primer sets for quantitative gene expression analysis of 13 species including human, mouse, rat, Arabidopsis, Drosophila, and C. elegans genes. These assays let you quickly, easily, and affordably perform precise gene expression studies using real-time PCR. TaqMan®-based assays provide unmatched sensitivity, specificity, and reproducibi…

rEVAlution 2X qPCR Master Mix is a ready to use hot-start qPCR master mix with EvaGreen® and FlashTaq, a hot start Taq polymerase. A 25μM ROX reference dye is supplied separately for use with some real time instruments. The master mix is optimized to work with both fast cycling conditions and regular three step cycling conditions. Available in 3 pack sizes: 100 20µL Reactions, 500 20µl reactions and 1,000 20µl reactions.

Lateral flow DNA detection

Primer and probe premixed assays for analyzing gene expression in any species using fluorescently labeled 5' nuclease probes

1 ml, one-step RT-PCR kit, includes 2x one-step SYBR Green reaction mix (Taq polymerase, buffers, ROX normalization dyes), iScript reverse transcriptase, and nuclease-free water, for 100 x 20 µl reactions

SensiFAST™ Probe Hi-ROX Kit and SYBR® Hi-ROX Kit have been developed for fast, highly reproducible real-time PCR and has been validated on commonly used real-time PCR instruments. A combination of the latest advances in buffer chemistry and PCR enhancers, together with a hot-start DNA polymerase, ensures that SensiFAST Kits delivers fast, highly-specific and ultra-sensitive real-time PCR.SensiFAST™ Probe Hi-ROX Kit has been d…

2 ml (2 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX normalization dyes, for 200 x 20 µl reactions

SingleShot™ Probes One-Step Cell Lysis and RT-qPCR Analysis Kit, 100 x 50 µl reactions

Rapid, sensitive and precise dye-based qPCR detection and quantitation of target DNA and cDNA sequences

Premix Ex Taq (Probe qPCR) Master Mix

TaqPath™ 1-Step Multiplex Master Mix is a single-tube, 4X RT-qPCR master mix that provides for sensitive, reproducible detection of up to four different RNA (or DNA) targets in a single multiplex reaction

Real-time PCR-based gene expression profiling.

TaqMan Universal DNA Spike-In Control serves as an exogenous process control of a known concentration that is spiked into a sample at specific stages throughout the qPCR process. 

Alkali Scientific offers Radiant™ Green Hi-ROX qPCR Kits, a high-performance 2X ready-to-use real-time PCR reagents designed for best-in-class quantitative PCR. Radiant™ Green Hi-ROX qPCR Kits are engineered for robust real-time PCR with earlier quantification cycle values (Ct), industry-leading sensitivity (increased limit of detection) and exceptional speed (rapid extension rates). The proprietary buffer system allows for hi…

SingleShot™ SYBR® Green Cell Lysis and RT-qPCR Kit, 100 x 50 µl reactions

25 ml (5 x 5 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX normalization dyes, for 2,500 x 20 µl reactions

1,000 x 20 µl reactions, 10 ml (2 x 5 ml) 2x supermix, contains Sso7d fusion polymerase in an advanced buffer formulation

Freeze dried qPCR reagents stored and shipped at ambient temperatures

Smart One RT-PCR Master Mix is designed for all applications that require Reverse Transcription of RNA into cDNA and a subsequent PCR amplification. Only primers and sample RNA have to be added to the mix

qPCRBIO Probe 1-Step Go is a universal probe kit designed for fast, highly specific and ultra-sensitive probe-based real-time RT-PCR.

Easy-to-use and flexible gene expression master mix for real-time PCR Features of PowerTrack SYBR Green Master Mixes include: Two-color tracking dye system indicates where pipetting has occurred Broad primer Tm and primer concentration compatibility allows flexibility in qPCR reaction setup with minimal optimization Superior specificity and tight reproducibility in Ct values over a broad dynamic range improve data…