TaqPath™ 1-Step Multiplex Master Mix
TaqPath™ 1-Step Multiplex Master Mix is a single-tube, 4X RT-qPCR master mix that provides for sensitive, reproducible detection of up to four different RNA (or DNA) targets in a single multiplex reaction
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Reliable, sensitive, and easy to use—TaqPath™ 1-Step Multiplex Master Mix (No ROX) has become a staple in our RT-qPCR workflows. Great performance, especially for multiplex assays with tricky samples.
Gene expression
TaqPath™ 1-Step Multiplex Master Mix (No ROX) has been highly effective in our multiplex RT-qPCR workflows. It consistently delivers sensitive and reproducible detection of low-copy RNA targets, even in complex clinical samples. The 4X concentration allows for greater sample input, and its inhibitor tolerance has been a game-changer for challenging matrices. Overall, it’s a reliable and efficient solution for high-throughput diagnostic applications.
Review Date: 27 Oct 2025 | Thermo Fisher Scientific
Great results with this master mix
Microbiology
The effectiveness is really good and it is easy to use.
Review Date: 22 Oct 2021 | Thermo Fisher Scientific
The assay is very robust, we can save a lot of time and money.
RT PCR for high Throughput Screening
By using this one-step master mix, we can have up to 4 gene expression with 4 different dyes in one reaction. The assay is very robust, we can save a lot of time and money.
Review Date: 17 Dec 2018 | Thermo Fisher Scientific
The best RT PCR multiplex Master Mix.
RT PCR High throughput Screening
We had tested different 1 step master mix from different vendors, the best one is TaqPath 1 Step Multiplex Master Mix. By using this master mix we can add up to 4 different gene expressions in one reaction. The result is very robust, it save time and money.
Review Date: 21 Nov 2018 | Thermo Fisher Scientific
TaqPath™ 1-Step Multiplex Master Mix is a single-tube, 4X RT-qPCR master mix that provides for sensitive, reproducible detection of up to four different RNA (or DNA) targets in a single multiplex reaction. Particularly useful for RNA virus detection and high throughput gene expression workflows, the mix includes thermostable MMLV reverse transcriptase, dNTPs, UNG, MUSTANG PURPLE™ dye (passive reference), and thermostable Fast DNA polymerase, all in a single tube.
This single-tube, 4X format facilitates easy reaction set up—just add user-supplied assay and sample. With reproducible performance even in the presence of inhibitors, coupled with stringent quality control to minimize lot-to-lot variation, TaqPath 1-Step Multiplex Master Mix, is a superior choice for your multiplex diagnostic testing or development needs.
Features of the TaqPath 1-Step Multiplex Master Mix include:
- High sensitivity to detect low-copy targets with reproducible CT results
- Broad dynamic range with both RNA and DNA targets
- Optimized for multiplexing up to 4 targets simultaneously with MUSTANG PURPLE reference dye
- Tolerant of inhibitors commonly found in clinical samples
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