Thermo Scientific™ SureTect™ Salmonella species PCR Assay
Rapidly detect Salmonella species in food and environmental samples: The Thermo Scientific™ SureTect™ Salmonella Species PCR Assay offers a rapid real-time PCR method for the enrichment, detection and confirmation of Salmonella species in food, animal feed products, including large sample sizes, and primary production samples (PPS).
Great results!
Pathogen detection
The method is relatively faster and easier to use. It provides a user-friendly software, less preparation time and lower risk of contamination during pathogen detection. The kit is validated by both AOAC and AFNOR and can be easily verified in the laboratory. It makes our work easier but still with high accuracy and sensitivity.
Review Date: 15 May 2024 | Thermo Fisher Scientific
Detect Salmonella species in food and environmental samples with the Thermo Scientific™ SureTect™ Salmonella Species PCR Assay which incorporates optimized primer and probe components for highly-specific detection of Salmonella species.
The assay's workflows have been validated for a broad range of human foods, pet food, including large test portions, and environmental samples by AOAC International and ISO 16140-2:2016 according to NF VALIDATION™ by AFNOR Certification and by MicroVal™ Certification. The assay is also certified for primary production samples by NF Validation™ by AFNOR Certification.
The new SureTect assay format incorporates color-coded plastics with orientation markings and breakable strip format and colored reagents and pierceable seals to enable an optimized workflow for maximum laboratory efficiency and productivity.
The SureTect System is an optimized solution combining speed and performance in an easy-to-use, cost-effective platform—giving you results you can trust.
Simple
- Easy-to-use with single enrichment step and pre-dispensed reagents
- Target-specific colored assay plastics
- Clearly oriented lysis and assay trays
- Color indicator in lysis reagent reducing possibility for human error
Fast and Flexible
- Option to automate the lysis and PCR sample preparation
- Optimized workflow delivers on-screen results in as little as 10 to 24 hours
- Maximum flexibility for operational efficiency
- Test up to 96 samples in each run
- Choice of pierceable seals or caps
- Detect multiple targets in the same run with universal PCR protocols across the range
Brochures
Rapid Salmonella detection and serotyping in poultry
In this product brochure, Thermo Fisher Scientific demonstrates how the Thermo Scientific™ SureTect™ Salmonella species Multiplex PCR Assay and Thermo Scientific™ SureTect™ Salmonella Infantis PCR Assay offer a complete, automatable solution for the detection of Salmonella spp. and identification of the top three serotypes in poultry products.
SureTect Real-Time PCR range for food pathogen detection
In this brochure, Thermo Fisher Scientific showcases the SureTect Real-Time PCR System and its full range of PCR solutions for food safety, authenticity, and quality testing.
Rapid and accurate detection of foodborne pathogens with the SureTect PCR range
In this brochure, Thermo Fisher Scientific showcases its SureTect Real-Time PCR System and portfolio of SureTect PCR Assays. The SureTect range is designed to provide accurate, rapid pathogen testing for a broad range of food, ingredients and environmental samples including; dairy, meat & poultry, seafood, produce, infant formula, chocolate samples, and more.
Food microbiology testing methods: Salmonella species
Effective detection and confirmation methods for Salmonella are crucial for public health and food safety. In this guide, Thermo Fisher Scientific illustrates a simple outline of the ISO reference method: ISO 6579-1:2017 (Horizontal method for the detection of Salmonella species), including information about Thermo Scientific products that can be used according to the method. The guide also details alternative methods, validated according to ISO 16140-2:2016 standard, that are available from Thermo Fisher Scientific.
SureTect Salmonella species PCR Assay workflow NF VALIDATION: ISO 16140-2:2016
The Thermo Scientific™ SureTect™ Salmonella species PCR Assay workflow has been certified by NF VALIDATION for the detection of Salmonella species from meat, dairy, seafood, vegetables, raw beef meats with and without aromatics, production environment samples and powdered infant formula samples. This application note gives a summary of the validation studies performed to gain NF VALIDATION.
SureTect Salmonella Species PCR Assay method comparison against ISO 6579-1:2017
In this application note, extended methods for the Thermo Scientific™ SureTect™ Salmonella species PCR Assay are compared against the International Organization for Standardization (ISO) 6579-1:2017 for a representative range of matrices.
Salmonella detection from large milk powder samples
Due to their ability to survive heating and desiccation, Salmonella and Cronobacter contamination is a concern for powdered infant formula (PIF), milk powders and their individual ingredients. In this application note, two studies are presented that evaluate the performance of the SureTect Salmonella species PCR Assay for the detection of Salmonella serovars from 375 g samples of milk powders, in comparison to ISO 6579-1:2017.
Use of an antibiotic-free enrichment medium for detection of Salmonella in cheese samples
In this application note, the performance of the SureTect Salmonella species assay is compared against the ISO reference method for detecting Salmonella from cheese samples using an enrichment medium without novobiocin.
Validation of the SureTect Salmonella species PCR Assay using the Applied Biosystems QuantStudio 5 PCR Instrument
This study demonstrates that the Thermo Scientific SureTect Salmonella species PCR Assay combined with the QuantStudio 5 Food Safety System is a suitable method for the detection of Salmonella species from a variety of food and environmental surface samples.
AOAC-RI PTM and NF validation of the Thermo Scientific SureTect Salmonella species PCR Assay
This poster outlines AOAC-RI PTM and NF VALIDATION studies that demonstrate that the SureTect Salmonella species PCR Assay Workflow, including the QuantStudio 5 Food Safety System, is a suitable method for the detection of Salmonella species in a broad range of food and environmental surfaces, with superior or equivalent performance to the reference method.
Reaching new heights in food safety testing using automated PCR workflows
Tuesday, September 30, at 15:00 BST | 16:00 CEST | 10:00 EDT | 07:00 PDT
Food safety testing laboratories are under growing pressure to do more with less. As testing volumes surge, labs must deliver accurate and regulatory compliant results, while navigating staffing shortages and limited resources. These challenges are further compounded by increasing sample sizes and throughput demands.
In this webinar, meet with Dr. Salman Zeitouni and Jani Holopainen to explore real-world examples of how automation is driving transformation in food safety testing laboratories. See how labs are leveraging automated solutions to enhance accuracy and efficiency, reduce human error, and build resilient testing capabilities.
Key learning objectives:
- Explore the drivers behind automation of molecular workflows in food safety testing laboratories
- Understand key considerations and common challenges in adopting lab automation
- Discover real-world examples of successful automation implementation in microbiology labs
- Gain insight into how automated molecular workflows can support lab scalability, traceability, and long-term resilience to build a future lab today.
Who should attend?
- Laboratory professionals, including food microbiologists, lab managers, and technical service directors.
- Quality, safety, and regulatory leaders, such as quality managers, operations directors, and compliance professionals.
Certificate of attendance
If you attend the live webinar, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
If you view the on-demand webinar, you can request a certificate of attendance by emailing editor@selectscience.net.
Screen for pathogens in four simple steps
In this video, discover how using the SureTect Real-Time PCR System you can quickly and accurately detect foodborne pathogens in a broad range of foods and associated samples, in just four simple steps.
It's easy to be sure with the SureTect PCR Pathogen Detection System
In this video, learn how the SureTect workflow can provide all the information you need for food safety, authenticity, and quality testing, using a system that is rapid, simple to implement, and easy to run on a day-to-day basis.
Latest validation offers food laboratories additional layer of confidence when testing for Salmonella
The Thermo Scientific SureTect Salmonella Species PCR Assay receives approval by AOAC International for a broad range of foods
Top tools and new techniques to ensure food quality and fight fraud
Discover the latest advances in food quality testing and authenticity determination
Thermo Scientific launches SureTect PCR Assays for expanded foodborne pathogen analysis
New assays for Campylobacter, STEC, Vibrio and Staphylococcus aureus enable more comprehensive pathogen testing for all food manufacturers
































