SsoAdvanced™ Universal SYBR® Green Supermix, 2,500 x 20 µl rxns, 25 ml (5 x 5 ml)
25 ml (5 x 5 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX normalization dyes, for 2,500 x 20 µl reactions
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Really reliable and easy-to-use SYBR Green mix for new and advanced qPCR users.
quantitative PCR (qPCR)
Our lab has been consistently using Bio-Rad's SsoAdvanced Universal SYBR Green Supermix on our BioRad CFX384 Real Time for nearly 6 years without issue. The supermix is incredibly easy to prepare with oligos. Furthermore, it's stability makes it easier to prepare multiple plates ahead of time and run back-to-back when our CFX384 is in high demand.
Review Date: 27 Oct 2025 | Bio-Rad
SsoAdvanced Universal SYBR Green Supermix is a high-performance real-time PCR supermix based on Bio-Rad's patented* Sso7d fusion protein technology. This supermix is formulated for a wide range of real-time PCR applications and for use with all ROX dependent or independent real-time PCR systems. The dsDNA binding protein, Sso7d, stabilizes the polymerase-template complex, providing superior inhibitor tolerance, increased processivity, specificity, and greater speed without affecting PCR sensitivity, efficiency, or reproducibility.
SsoAdvanced Universal SYBR Green Supermix lets you:
- Use any real-time PCR system — the universal reference dye in this supermix enables ROX normalization of qPCR data regardless of the ROX level requirements of the qPCR system
- Achieve superior real-time PCR results under various conditions — our robust formulation delivers consistent performance in fast cycling across a broad range of reaction conditions, primer concentrations, and temperature ranges
- Increase your qPCR sensitivity and efficiency of detection from compromised samples — the Sso7d fusion polymerase has increased resistance to a wide variety of PCR inhibitors, which helps provide better sensitivity and overall performance
- Decrease time to results without compromising qPCR data quality — the Sso7d fusion polymerase and optimized buffer together provide rapid polymerization kinetics and instant polymerase activation
- Obtain better results with Bio-Rad's PrimePCR™ assays — real-time PCR assays are expertly designed and wet-lab validated to ensure optimal assay performance
Instrument Compatibility
SsoAdvanced Universal SYBR Green Supermix is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:
* BSA must be added according to instrument specifications
Applications and Uses of SsoAdvanced Universal SYBR Green Supermix
- qPCR/real-time PCR
- Gene expression analysis
- Pathway analysis
- Absolute quantification
- Chromatin immunoprecipitation (ChIP) qPCR
- Mutation detection
- Pathogen detection
- Viral detection (load)
- Characterization of genetically modified organisms (GMO)
- Genetic profiling
Related Products
We have developed SsoAdvanced universal probes supermix , which is available in several formats (200–5,000 reactions).
Bio-Rad offers a complete workflow solution for real-time PCR, including:
- RNA isolation kits
- Reverse transcription kits
- Real-time PCR supermixes
More Information
To ensure that your qPCR reactions are reproducible and reliable, follow our step-by-step design and optimization protocols . We also provide insights into analysis of qPCR data and troubleshooting reactions .
* U.S. patents 6,627,424; 7,541,170; and 7,560,260.
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