Practical workflow for RT-qPCR experiments aligned with MIQE guidelines

12 May 2026

This resource outlines a practical approach to designing and performing RT‑qPCR experiments in accordance with MIQE guidelines to ensure high‑quality, reproducible gene expression data. It details key steps across the workflow, including experimental design, RNA extraction and quality control, reverse transcription, primer design, and assay validation. By emphasising standardisation, proper controls, and robust data analysis, the guide provides researchers with a framework to improve consistency, reliability, and comparability of RT‑qPCR results.

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