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CFX96 Touch™ Deep Well Real-Time PCR Detection System


Modular thermal cycler platform, includes C1000 Touch™ thermal cycler chassis, CFX96 Deep Well™ optical reaction module, CFX Manager™ software, qbasePLUS software license, cables

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Average Rating 4.7

|11Scientists have reviewed this product

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Easy to use, easy to customize as well


Average Rating 4.7

Application Area:

Copy number quantification

Easy to use and reproducible results. Only issue I had was some pre programmed setups were not easy to modify.

Review Date: 28 Feb 2023 | Bio-Rad

Great instrument ,very dynamic and durable


Average Rating 3.3

Application Area:

Qualitative and Quantitative PCR

The equipment is versatile and has been validated with a lot of Reagents kits thus it is applicable for various systems,However the standard curve cannot ne exported ans used for various runs when running viral loads

Review Date: 26 Jan 2023 | Bio-Rad

Great result


Average Rating 5.0

Application Area:

Molecular genetics

Value for money

Review Date: 17 Jun 2022 | Bio-Rad

Reliable, easy to use PCR instrument


Average Rating 4.0

Application Area:

Molecular diagnostics

The instrument is very easy to use, relative to other PCR instruments. The software can be a bit clunky, but once you’ve learned it, it is reliable and not bad. Where Bio-Rad really shines is their customer service. Their technical help is fantastic and easy to get in touch with - no auto responses, actual people answer the phone.

Review Date: 20 May 2022 | Bio-Rad

A must buy user-friendly, cost effective product


Average Rating 5.0

Application Area:

Expression profiling from tissue samples

The product is cost-effective, user-friendly, and highly sensitive. I was impressed with the aftersales service and customer support. I have been recommending it to my new Institute, where I work currently, and are in process of purchasing one. The instrument comes with a touch screen and a highly sensitive detection system compatible with SYBR and FAM. The software allows the analysis of the data and can be imported into multiple formats. Has a nice gradient facility available as well. A must-buy product for any molecular-bio Lab.

Review Date: 29 Apr 2022 | Bio-Rad

Reliable and easy to use.


Average Rating 5.0

Application Area:

Plant pathogen identification and DNA amplification

Bio-Rad gave us the best options for our first qPCR and knowing the brand from previous labs it was a very easy choice.

Review Date: 1 Apr 2022 | Bio-Rad

Easy and high quality


Average Rating 4.7

Application Area:

Analyze gene expression

The perfect real time machine to analyze gene expression. It is easy to use, user friendly and with high quality. The led system is perfect and allows the multiple analysis of different colors in parallel

Review Date: 1 Apr 2022 | Bio-Rad

Great results!


Average Rating 5.0

Application Area:

Real time PCR for Clinical samples

The product gives accurate results. Machine is user friendly and easy to handle.

Review Date: 30 Mar 2022 | Bio-Rad

Accurate, precise, and reproducible results


Average Rating 5.0

Application Area:

Plant pathogen testing

The qPCR uses a multi-channel fluorescence detector therefore, can detect up to 6 targets in a single reaction. It is a cost-effective and time-saving option to set up in-house assays for any cannabis producer.

Review Date: 4 Feb 2022 | Bio-Rad

Great for routine use in PCR lab.


Average Rating 5.0

Application Area:

Clinical microbiology - clinical virology

Easy plate set-up for the run, instrument is relatively quiet, fast, a lot of channels/dyes, simple reading of results.

Review Date: 15 Jun 2021 | Bio-Rad

Use the CFX96 Deep Well Real-Time PCR Detection System for precise, reproducible real-time PCR. The system is composed of the CFX96 Deep Well Optical Reaction Module and the C1000 Touch Thermal Cycler Chassis. The module is a member of the family of interchangeable reaction modules that can be used with the C1000 Touch Thermal Cycler Chassis for PCR and real-time PCR. All modules can be quickly inserted and removed from the cycler chassis without tools.

Features and Benefits

  • Deep wells for large volumes
  • Detection of up to 5 targets per well, plus a channel dedicated to singleplex FRET
  • Protocol autowriter that generates an optimal protocol for your reaction components
  • Thermal gradient that identifies optimal annealing temperature in a single run

The CFX96 Deep Well Real-Time PCR Detection System has six independently controlled thermal electric units providing even, precisely controlled temperatures at all times during the run, including ramping. The reduced mass of the honeycomb block provides fast ramping and reduced settling time (time to achieve thermal uniformity).

The optical system, with six filtered LEDs and six filtered photodiodes, collects data from all wells, detecting up to five targets per well. For single-color FAM and SYBR® Green I, the fast scan option reads single-channel fluorescence in all 96 wells in just 3 seconds. A channel with an LED filter–photodiode combination is dedicated to FRET singleplex experiments.

The large touch-screen interface has intuitive graphical programming and displays data traces in real time. The programmable thermal gradient feature can test eight temperatures at once to quickly determine the optimal annealing temperature, and the protocol autowriter quickly optimizes reactions, generating an optimal protocol based on your polymerase, primers, and product length.

Product Components

  • C1000 Touch Thermal Cycler Chassis ( 1841100 )
  • CFX96 Deep Well Optical Reaction Module ( 1840197 )
  • USB cable
  • Instruction manual

Configuration Options

The following modules can be used with the C1000 Touch Thermal Cycler Chassis:

*Starter package contains SsoAdvanced™ Universal SYBR® Green Supermix ( 1725270 ), Microseal® 'B' Adhesive Seals ( MSB1001 ), and Hard-Shell® Low-Profile Thin-Wall 96-Well Skirted PCR Plates ( HSP9601 ) or Hard-Shell Thin-Wall 384-Well Skirted PCR Plates ( HSP3805 ).

The CFX96 Deep Well Real-Time PCR Detection System can be configured with other PCR instruments, allowing stand-alone or software control. Instrument permutations allow up to four different CFX Connect™, CFX96, or CFX384 Systems to be controlled by one PC.

Combine any two CFX Real-Time PCR Detection Systems with the CFX Automation System II , our robotic plate handler, to create a high-throughput, automated real-time PCR system with a compact footprint.

Service Plans and Validation Tools for CFX Systems

  • Ensure that your real-time PCR systems are functioning optimally with Bio-Rad Expert Care Service ; choose from several service plans to keep your CFX Automation System II up and running its best
  • Certify system installation and validate system performance with CFX System IQ/OQ Services and CFX Automation System II IQ/OQ Services
  • Verify the performance of your CFX Systems with Thermal Validation Services and the CFX Qualification Plates

Related Products

  • PX1™ PCR Plate Sealer
  • PCR plastics optimized for CFX Real-Time PCR Detection Systems:
    • Hard-Shell 96-Well Low Profile Skirted PCR Plates including barcoded plates ( HSP9955 )
    • Microseal 'B' Adhesive Seals ( MSB1001 )
    • Optically Clear Heat Seals ( 1814030 )
  • CFX Maestro Software, Security Edition , for compliance with U.S. FDA 21 CFR Part 11 regulations
  • CFX Qualification Plate for performance verification, 96-well ( 1845098 )

Related Categories

  • Real-Time PCR Detection Systems
  • PCR Reagents and qPCR Reagents
  • PCR Plastic Consumables
  • PCR Seals
  • PCR Analysis Software

Online Resources

  • Real-Time PCR Detection Systems Selection Chart
  • qPCR/Real-Time PCR
  • qPCR Instrumentation

Product Overview