iScript™ cDNA Synthesis Kit, 100 x 20 µl rxns
100 x 20 µl reactions, reverse-transcription reagent kit, includes 5x iScript reaction mix, iScript reverse transcriptase, nuclease-free water
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Great
cDNA synthesis
Great product. I always downscale to half but still get enough cDNA
Review Date: 19 Jun 2023 | Bio-Rad
Use the iScript cDNA Synthesis Kit for first-strand cDNA synthesis for real-time qPCR. This two-tube kit can accommodate dilute RNA samples.
Key Features and Benefits
- Up to 15 µl of RNA in a 20 µl cDNA synthesis reaction
- Combined oligo(dT) and random primers provide unbiased representation of 5' and 3' regions
- Fast 26 min cDNA synthesis protocol
- Linear range of 1 µg–1 pg total input RNA using RNase H+ MMLV reverse transcriptase
See the iScript cDNA Synthesis Kit product page for more information and documentation.
Related Products
- View all cDNA Synthesis Kits
- Real-Time PCR Supermixes and Kits
- PrimePCR™ PCR Primers & Assays
Related Categories
- PCR and qPCR Reagents
- Real-Time PCR Detection Systems
- PCR Plastic Consumables
- Instrument Validation Tools and Services
Online Resources
- qPCR/Real-Time PCR
- Genomics
Practical workflow for RT-qPCR experiments aligned with MIQE guidelines
This resource outlines a practical approach to designing and performing RT‑qPCR experiments in accordance with MIQE guidelines to ensure high‑quality, reproducible gene expression data. It details key steps across the workflow, including experimental design, RNA extraction and quality control, reverse transcription, primer design, and assay validation. By emphasising standardisation, proper controls, and robust data analysis, the guide provides researchers with a framework to improve consistency, reliability, and comparability of RT‑qPCR results.
