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iScript™ cDNA Synthesis Kit, 25 x 20 µl rxns

Bio-Rad1708890Available: Worldwide

25 x 20 µl reactions, reverse-transcription reagent kit, includes 5x iScript reaction mix, iScript reverse transcriptase, nuclease-free water

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Average Rating 4.8

|4Scientists have reviewed this product

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Desirable results are obtained by using this cDNA synthesis kit


Average Rating 5.0

Application Area:

Liver tissue samples

The cDNA synthesis kit is totally fantastic and easy to use

Review Date: 17 Dec 2021 | Bio-Rad

Easy to use, great yield every time, painless


Average Rating 4.7

Application Area:

RT-PCT, qRT-PCR, etc

Very easy to use, works every time, almost effortless. Kit shelf life is extremely long, never has a problem, best bang for the money!

Review Date: 3 Nov 2017 | Bio-Rad

Great for most cDNA for qPCR


Average Rating 4.7

Application Area:

cDNA for qPCR

Great kit for the synthesis of cDNA, is very consistent. However, if you are utilizing a small amount of RNA this kit isn't that great. Would recommend a more sensitive RT.

Review Date: 15 Jan 2016 | Bio-Rad

Average Rating 5.0

Review Date: 29 Sept 2015 | Bio-Rad

Use the iScript cDNA Synthesis Kit for first-strand cDNA synthesis for real-time qPCR. This two-tube kit can accommodate dilute RNA samples.

Key Features and Benefits

  • Up to 15 µl of RNA in a 20 µl cDNA synthesis reaction
  • Combined oligo(dT) and random primers provide unbiased representation of 5' and 3' regions
  • Fast 26 min cDNA synthesis protocol
  • Linear range of 1 µg–1 pg total input RNA using RNase H+ MMLV reverse transcriptase

See the iScript cDNA Synthesis Kit product page for more information and documentation.

Related Products

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Related Categories

  • PCR and qPCR Reagents
  • Real-Time PCR Detection Systems
  • PCR Plastic Consumables
  • Instrument Validation Tools and Services

Online Resources

  • qPCR/Real-Time PCR
  • Genomics

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