Aurum™ Total RNA Mini Kit
DNA-free RNA isolation kit, includes RNA binding columns, capless wash and capped microcentrifuge tubes, lyophilized DNase I, RNase-free reagents, for 50 preps
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DNA-free RNA isolation kit, includes RNA binding columns, capless wash and capped microcentrifuge tubes, lyophilized DNase I, RNase-free reagents, for 50 preps
Practical workflow for RT-qPCR experiments aligned with MIQE guidelines
This resource outlines a practical approach to designing and performing RT‑qPCR experiments in accordance with MIQE guidelines to ensure high‑quality, reproducible gene expression data. It details key steps across the workflow, including experimental design, RNA extraction and quality control, reverse transcription, primer design, and assay validation. By emphasising standardisation, proper controls, and robust data analysis, the guide provides researchers with a framework to improve consistency, reliability, and comparability of RT‑qPCR results.
The past, present, and future of digital PCR
This resource outlines a practical workflow for conducting RT‑qPCR experiments in alignment with MIQE guidelines to ensure robust, reproducible gene expression data. Covering key steps from experimental design and RNA extraction to assay validation and data analysis, it highlights critical factors such as RNA quality, primer design, reaction efficiency, and use of appropriate controls and replicates. By following these standardized practices, researchers can improve data consistency, reliability, and comparability across studies.
High-Throughput Automated Extraction of RNA Using the Aurum™ Total RNA 96 Kit
RNA extractions for use in qPCR assays are labor-intensive, time-consuming procedures that are now amenable to automation. This raises the question of whether commercially available RNA extraction kits that have been designed for low- to medium-throughput extractions using centrifugation or filtration-based methods can be used in automated high-throughput procedures. This study evaluates the use of the Aurum total RNA 96 kit in a fully automated RNA extraction process.
