The past, present, and future of digital PCR

This resource outlines a practical workflow for conducting RT‑qPCR experiments in alignment with MIQE guidelines to ensure robust, reproducible gene expression data. Covering key steps from experimental design and RNA extraction to assay validation and data analysis, it highlights critical factors such as RNA quality, primer design, reaction efficiency, and use of appropriate controls and replicates. By following these standardized practices, researchers can improve data consistency, reliability, and comparability across studies.