iScript™ cDNA Synthesis Kit, 500 µl (170-8891BUN)
500 x 20 µl reactions, includes 5x reverse-transcription reaction mix, iScript reverse transc
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Use the iScript cDNA synthesis kit for first-strand cDNA synthesis for gene expression analysis using real-time qPCR.
This two-tube kit is optimized to yield sensitive, unbiased representation over a broad dynamic range with minimal setup and reaction time.
- 2 tubes (iScript reaction mix and iScript reverse transcriptase) for faster setup
- Format allows for up to 15 µl of RNA sample in a 20 µl cDNA synthesis reaction
- 500 x 20 µl reactions
- RNaseA inhibitor protects RNA during setup and reverse transcription
- Fast 40 min cDNA synthesis protocol
Practical workflow for RT-qPCR experiments aligned with MIQE guidelines
This resource outlines a practical approach to designing and performing RT‑qPCR experiments in accordance with MIQE guidelines to ensure high‑quality, reproducible gene expression data. It details key steps across the workflow, including experimental design, RNA extraction and quality control, reverse transcription, primer design, and assay validation. By emphasising standardisation, proper controls, and robust data analysis, the guide provides researchers with a framework to improve consistency, reliability, and comparability of RT‑qPCR results.
