Products & ReviewLife Sciences

GoTaq Real-Time PCR Systems

Promega Corp.A6001, A6002

The GoTaq®Real-Time PCR Systems are ready-to-use 2X master mixes optimized for quantitative PCR in dye (using BRYT(R) Green Dye) or hydrolysis probe detection format. The 1-Step and 2-Step RT-qPCR Master Mixes contain GoScript™ Reverse Transcriptase for efficient first-strand cDNA synthesis. GoTaq Real-Time PCR Systems Features & Benefits: Higher fluorescence, often resulting in earlier Cqs Direct su…

4.7/5.0

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Average Rating 4.7

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Average Rating 4.7

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DNA production

Very easy to use, easy instruction and high yield of product.

Review Date: 15 Jul 2022 | Promega Corp.

The GoTaq®Real-Time PCR Systems are ready-to-use 2X master mixes optimized for quantitative PCR in dye (using BRYT(R) Green Dye) or hydrolysis probe detection format.

The 1-Step and 2-Step RT-qPCR Master Mixes contain GoScript™ Reverse Transcriptase for efficient first-strand cDNA synthesis.

GoTaq Real-Time PCR Systems Features & Benefits:

Higher fluorescence, often resulting in earlier Cqs
Direct substitute for SYBR® Green I products
Enhanced stability for automated setup
Choose from qPCR, 1-Step or 2-Step RT-qPCR options
Compatible with both fast and standard cycling methods
Sensitive quantitation on most real-time instruments

Application NoteLife Sciences

RNA Quantification Using the GoTaq® RT-qPCR Systems

Promega GoTaq® products for RNA quantification offer two dye-based systems for accurate, sensitive, broad-range quantification of RNA targets. This application note demonstrates the successful amplification of 11 differentially expressed mRNA targets using the GoTaq® 1-Step RT-qPCR System. One of these targets was also amplified to demonstrate equivalent performance between the GoTaq® 1-Step and GoTaq® 2-Step RT-qPCR Systems. It was found that reactions with the GoTaq® RT-qPCR Systems have a higher change in fluorescence and earlier Cq values than other RT-qPCR systems.

2 May 2017

Product Overview

GoTaq Real-Time PCR Systems - Promega Corp. - Life Sciences

GoTaq Real-Time PCR Systems

4.7/5.0

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