Steady-Glo ® Luciferase Assay System
High-throughput quantitation of firefly (Photinus pyralis) luciferase expression in mammalian cells is commonly performed by batch processing of 96- and 384-well plates. Steady-Glo® Luciferase Assay System(a,b,c) is designed for this purpose by providing long-lived luminescence when added to cultured cells. The homogeneous assay provides signal half-lives of over 5 hours in commonly used cell culture media without prior sample…
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Best assay for luciferase.
Analyze luciferase activity
Good reproducible results, easy to use in only one step.
Review Date: 25 Oct 2019 | Promega Corp.
Great assay.
Quantification within a pharmocological assay
This product gave really good, reliable results in our reader. I had no issues using it, as the protocol was really simple.
Review Date: 27 Jun 2019 | Promega Corp.
Reproducible and Robust results-what you ask for!!!
Gene expression quantification
Ready to use product with easy preparation for quantifying gene expression levels in a reproducible manner. The great thing about Steady-Glo is the stability of signal over long time , more than enough for measurement period.
Review Date: 1 Nov 2017 | Promega Corp.
Review Date: 3 Apr 2008 | Promega Corp.
A good kit to use in combination with proprietary vectors.
Review Date: 12 Oct 2007 | Promega Corp.
High-throughput quantitation of firefly (Photinus pyralis) luciferase expression in mammalian cells is commonly performed by batch processing of 96- and 384-well plates. Steady-Glo® Luciferase Assay System(a,b,c) is designed for this purpose by providing long-lived luminescence when added to cultured cells. The homogeneous assay provides signal half-lives of over 5 hours in commonly used cell culture media without prior sample processing. Throughput rates of several thousand samples per hour may be achieved with high reproducibility under standard laboratory conditions.
The P450-Glo CYP2B6 Assay: A Rapid and Selective Assay for Measuring CYP2B6 Induction and Inhibition
Promega has developed a luminogenic CYP2B6 assay for biochemical CYP2B6 inhibition and cell-based CYP2B6 induction studies. In this application note, Promega presents the new P450-Glo™ CYP2B6 luminogenic assay characterization and demonstrate its utility for measuring both CYP2B6 inhibition and induction.
Design and Validation of Bioluminescent Assays for 3D Cell Culture Models
This poster demonstrates the development of an improved reagent formulation for bioluminescent detection of ATP for measuring cell viability.
