Products & ReviewLife Sciences

GSH-Glo™ Glutathione Assay

Promega Corp.V6911, V6912

The GSH-Glo™ Glutathione Assay is a luminescence-based assay for the detection and quantification of glutathione (GSH). The assay is based on the conversion of a luciferin derivative into luciferin in the presence of glutathione, catalyzed by glutathione S-transferase (GST). The signal generated in a coupled reaction with firefly luciferase is proportional to the amount of glutathione present in the sample. Luminescence is gen…

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Average Rating 5.0

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Better than our in house kit.

Average Rating 5.0

Application Area:

Analyze GSH levels in cell culture

It has proven to be better than our assays in house. Our current methods have low fluorescence. This kit proved to pick up the slightest changes in my cells. We are continuing to optimize our protocols based upon Promega's GSH assay, and we will be doing business again.

Review Date: 24 Jan 2020 | Promega Corp.

Better than our protocol and reagents.

Average Rating 5.0

Application Area:

Analyze cell culture samples

It works better than our current methods. Thanks for a great product! We spent months trying to optimize our own protocol before we switched and tried this one. Doesn't disappoint.

Review Date: 26 Oct 2019 | Promega Corp.

Average Rating 5.0

Great one step assay for GSH.

Review Date: 6 Dec 2010 | Promega Corp.

The GSH-Glo™ Glutathione Assay is a luminescence-based assay for the detection and quantification of glutathione (GSH). The assay is based on the conversion of a luciferin derivative into luciferin in the presence of glutathione, catalyzed by glutathione S-transferase (GST). The signal generated in a coupled reaction with firefly luciferase is proportional to the amount of glutathione present in the sample. Luminescence is generated in less than one hour. The assay generates a stable luminescence signal and is simple, fast and easily adaptable to multiwell formats such as 96- and 384-well plates. This assay can be used for detection and quantification of GSH in cultured cells or in various biological samples.

Scientific PosterLife Sciences

Homogeneous Add-Mix-Read Assay for Total and Oxidized Glutathione

A wide variety of compounds can induce intracellular formation of reactive oxygen species (ROS) and the resulting cellular damage can eventually lead to apoptosis or necrosis. Measuring the ratio of reduced to oxidized glutathione (GSH/GSSG ratio) is a way to determine how such compounds alter the redox potential in the cell. GSH/GSSG-Glo allows for rapid, direct measurement of total and oxidized glutathione in cell-culture wells using low cell density.

27 Aug 2015

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GSH-Glo™ Glutathione Assay - Promega Corp. - Life Sciences

GSH-Glo™ Glutathione Assay

5.0/5.0

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