Dual-Glo® Luciferase Assay System
The Dual-Glo® Luciferase Assay System is a homogeneous reagent system that enables fast and simple quantitation of a stable luminescent signal from two reporter genes in a single sample. This convenient "add-and-read" system generates both firefly and Renilla luciferase luminescence signals from cells that have not been preconditioned or prelysed. With the Dual-Glo® System, internal controls can be established to minimize s…
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Works well.
Protein expression
We have been using the Dual-Glo assay system for years. It is highly sensitive and reproducible. The downside is the price tag.
Review Date: 16 Nov 2021 | Promega Corp.
Easy to use, reproducible results.
Analyze mammalian cell samples
Very easy to use, clear protocol, reproducible results in a convenient plate format.
Review Date: 31 Oct 2019 | Promega Corp.
Great product.
Analyze activation of reporter system in cell line
It was very easy to use to get the answer I wanted.
Review Date: 25 Oct 2019 | Promega Corp.
This kit is easy to use with great depletion of firefly signal.
Review Date: 28 Jan 2011 | Promega Corp.
Very expensive!
Review Date: 18 May 2009 | Promega Corp.
The Dual-Glo® Luciferase Assay System is a homogeneous reagent system that enables fast and simple quantitation of a stable luminescent signal from two reporter genes in a single sample.
This convenient "add-and-read" system generates both firefly and Renilla luciferase luminescence signals from cells that have not been preconditioned or prelysed. With the Dual-Glo® System, internal controls can be established to minimize sample variability by reducing false positive and false negative readings caused by nonspecific factors such as cytotoxicity. In the Dual-Glo® Luciferase Assay, the activity of the primary reporter is correlated with the effect of specific stimuli, and the activity of the co-transfected control reporter provides an internal control to normalize results.
The P450-Glo CYP2B6 Assay: A Rapid and Selective Assay for Measuring CYP2B6 Induction and Inhibition
Promega has developed a luminogenic CYP2B6 assay for biochemical CYP2B6 inhibition and cell-based CYP2B6 induction studies. In this application note, Promega presents the new P450-Glo™ CYP2B6 luminogenic assay characterization and demonstrate its utility for measuring both CYP2B6 inhibition and induction.
Miniaturized GloSensor cAMP Assay by Promega, Using the Echo® Liquid Handler
As biological assays used for HTS and uHTS are miniaturized, it is critical to maintain assay sensitivity while minimizing reagent volumes and maximizing throughput. The GloSensor cAMP Assay provides an extremely sensitive and easy to use, real-time luminescent assay format for the interrogation of over-expressed or endogenous GPCRs that signal via changes in the intracellular concentration of cAMP. This scientific poster demonstrates optimization of the GloSensor cAMP Assay in low-volume format using a stably transfected HEK293 cell line model expressing the melanocortin 4 receptor (MC4R) and a cAMP-sensing variant of firefly luciferase.
