Miniaturized GloSensor cAMP Assay by Promega, Using the Echo® Liquid Handler
26 Jul 2015As biological assays used for HTS and uHTS are miniaturized, it is critical to maintain assay sensitivity while minimizing reagent volumes and maximizing throughput. The GloSensor cAMP Assay provides an extremely sensitive and easy to use, real-time luminescent assay format for the interrogation of over-expressed or endogenous GPCRs that signal via changes in the intracellular concentration of cAMP. This scientific poster demonstrates optimization of the GloSensor cAMP Assay in low-volume format using a stably transfected HEK293 cell line model expressing the melanocortin 4 receptor (MC4R) and a cAMP-sensing variant of firefly luciferase.
Related Products
Request Quote for All Products
Nano-Glo® Dual-Luciferase® Reporter Assay System
Promega Corp.Switch Seamlessly from Your Current Dual-Luciferase® Assay!
Dual-Glo® Luciferase Assay System
Promega Corp.The Dual-Glo® Luciferase Assay System is a homogeneous reagent system that enables fast and simple quantitation of a stable luminescent signal from two reporter genes in a single sample. This convenient "add-and-read" system generates both firefly and Renilla luciferase luminescence signals from cells that have not been preconditioned or prelysed. With the Dual-Glo® System, internal controls can be established to minimize sample variability by reducing false positive and false negative readings caused by nonspecific factors such as cytotoxicity. In the Dual-Glo® Luciferase Assay, the activity of the primary reporter is correlated with the effect of specific stimuli, and the activity of the co-transfected control reporter provides an internal control to normalize results.