Gene Expression and Molecular Cloning Products & Reviews

The Agilent SurePrint G3 Human GE 8x60K Microarrays offer the same high sensitivity and accuracy provided by the earlier generation arrays but adds the benefits of extended coverage, greater throughput, and increased cost savings. The new SurePrint G3 Human GE 8x60K Microarrays and Human GE 4x44K v2 Microarrays are based on updated transcriptome databases for mRNA targets, while the SurePrint G3 arrays also include probes for…

Human iPSC-derived microglia expressing Cas9 for rapid gene knockout generation

Unleash the full power of a well-designed library. With Twist Bioscience, you are assured that the library you design contains the specific modifications, exactly where you want them, saving you valuable resources for the downstream screening process.

BioIVT is the leading provider of quality, highly characterized, ethically procured ADME research products, featuring custom preparation or extensive ready-to-ship inventory, large lot sizes and expert support.

Achieve ultra-high RNA yields with MEGAscript™ kits—ideal for in vitro or in vivo translation studies needing unlabeled or low specific activity RNA.

FDA approved Modern cellular and molecular biology poses the strictest requirements for the usage of sensitive samples such as living cells, nucleic acids or other cell components. With this ambition of quality in mind, Eppendorf offers a large selection of microcapillaries for the most varied applications. VacuTips capillaries and TransferTips® for intracytoplasmatic sperm injection (ICSI ) TransferTip (ES) for embryo…

NanoLuc® and NanoBiT® Luciferase Substrate for Animal Imaging Studies​

The ONE-Glo™ + Tox Assay combines luciferase assay chemistry with a cell viability marker to better understand reporter gene expression in the context of cell health. The assay uses a two-step, addition-only process to make these measurements in a single well of a plate, negating the need to run parallel assays. The first part of the assay is a nonlytic fluorescence assay (CellTiter-Fluor™ Cell Viability Assay) that measure…

The TnT® Quick Systems are convenient single-tube, coupled transcription/translation reactions for eukaryotic cell-free protein expression.   These cell-free expression systems combine the RNA Polymerase, nucleotides, salts, amino acids and Recombinant RNasin® Ribonuclease Inhibitor with the reticulocyte lysate solution to form a single TnT® Quick Master Mix. The TnT® Quick Coupled Transcription/Translation System is ava…

The Kazusa ORFeome Project was initiated to create a collection of human and mouse ORFs to enable the larger research community with a readily accessible, highly validated ORFeome. Kazusa DNA Research Institute (KDRI) is an academic Japanese research institute dedicated to genome-scale studies and the function of individual genes. KDRI is an active member of the global ORFeome Collaboration, and are experts in HUGE (Human Uni…

The TNT® Coupled Reticulocyte Lysate Systems offer researchers an alternative for eukaryotic cell-free protein expression: a single-tube, coupled transcription/translation system. The TNT® Lysate Systems greatly simplify the process and reduce the time required to obtain in vitro translation results.

Predesigned probe assays for gene expression analysis

Modulation of gene expression with modified oligos

The CD73 activity assay for HTS and hit-to-lead. Measure specific activity of CD73 in a simple mix-and-read format. The kit is designed for compound library screening and follow-up inhibitor discovery.

UltraScript® cDNA Synthesis Kit Separate Oligos contains all the components for efficient and sensitive cDNA synthesis over a broad range of RNA template concentrations. Anchored oligo(dT) and random hexamers are provided separately for user optimisation depending on the type of analysis needed.

UltraScript 2.0 cDNA Synthesis Kit Separate Oligos containins all the components required for fast, reliable and unbiased cDNA synthesis of the most challenging RNA sample types. Anchored oligo(dT) and random hexamers are provided separately for user optimisation depending on the type of analysis needed.

UltraScript® 2.0 cDNA Synthesis Kit is a 2-tube kit containing all the components required for fast, reliable and unbiased cDNA synthesis of the most challenging RNA sample types.

UltraScript® 2.0 Reverse Transcriptase is a highly thermostable reverse transcriptase designed for cDNA synthesis of the most challenging RNA sample types. This modified MMLV reverse transcriptase can be used with reaction temperatures of over 55 °C, giving improved specificity, higher cDNA yields and more full length cDNA product.

UltraScript® cDNA Synthesis Kit is an easy-to-use 2-tube system specifically developed to generate cDNA for use in real-time PCR. The kit provides unbiased, efficient and sensitive cDNA synthesis over a broad range of RNA template concentrations.

Fast-track your route to a modernized workflow with the only in vitro bioavailability assessment tool on the market

Amplify a CRISPR guide sequence derived from a CROP-style vector alongside your standard RNA Sequencing Library.

Digests linear RNA and Y-structure RNA, leaving circRNA, lariat intron RNA, and dsRNA intact. Ideal for isolating circRNA from total RNA preparations. 1 µg of RNase R digests 5 µg of linear RNA in 30 minutes.

FOPflash: Transfection grade T cell factor (TCF) reporter plasmid containing two full and one incomplete copy of the TCF binding site (mutated) followed by three copies in the reverse orientation, upstream of the Thymidine Kinase (TK) minimal promoter and Luciferase open reading frame. This plasmid serves as a negative control to TOPflash which contains wild type TCF binding sites.

TOPglow (TCF reporter plasmid): Transfection grade T cell factor (TCF) reporter plasmid containing four copies of the Tcf binding site (wt) upstream of E1B TATA box and Luciferase open reading frame.