INCOGNITO™ T7 ARCA 5mC- & Ψ-RNA Transcription Kit
Co-transcriptionally cap 5-methyl-cytidine- pseudouridine-containing, in vitro transcribed RNA with Anti-Reverse cap analog (ARCA). Produce up to 40 µg of capped 5mCΨ-RNA from 1 µg of template in 3 hours. Optimized for maximum transcript capping and yield. Induce lower innate immune responses in mammalian cells by using 5mCΨ-mRNAs.
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The INCOGNITO™ T7 ARCA 5mC- & Ѱ-RNA Transcription Kit is optimized for high-yield synthesis of 5-methyl-cytidine- pseudouridine-containing anti-reverse cap analog-capped RNA from an in vitro transcription reaction. A 3 hour, 20 µl reaction yields up to 40 µg of ARCA-5mCѰ-RNA from 1 µg of the 1.4 kb standard T7 control template DNA. Because ARCA contains a 3'-O-methyl group on the m7G nucleotide, ARCA can only be incorporated in the correct orientation at the 5' end of the RNA during an in vitro transcription/capping reaction. This is not true for the standard cap analog. Thus, ARCA incorporation results in the synthesis of capped RNA that is more efficiently translated in vivo than standard cap analog. Kit includes a limited non-exclusive, non-transferable label license to use the products purchased from CELLSCRIPT™ solely for purchaser's own internal laboratory research use. Product is for research use only (RUO).
