A-Plus™ Poly(A) Polymerase Tailing Kit
For post-transcriptional poly(A) tail addition to RNAs. Tail mRNAs in vitro to lengths equivalent to or greater than those found in nature.
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The A-Plus™ Poly(A) Polymerase Tailing Kit uses ATP as a substrate for template-independent addition of adenosine monophosphates to the 3'-hydroxyl termini of RNA. The standard protocol produces a poly(A)-tail length of ~150 bases on 40-60 µg of RNA, although by using an alternative modiified protocol tail lengths >300 bases can be achieved. Polyadenylation increases the stability of RNA in eukaryotic cells and enhances its ability to be translated after transfection or microinjection. A poly(A) tail is useful to provide a priming site for first-strand cDNA synthesis in certain applications, and can be used to end-label or quantify mRNA. Product is for research use only (RUO).
