INCOGNITO™ T7 mScript™ Ψ-mRNA Production System
All-in-one kit to produce pseudouridine-containing-mRNA (Ψ-mRNA). Combine the benefits of high-yield transcription with post-transcriptional capping and tailing. Produce large amounts of Ψ-mRNA with virtually 100% transcript capping. Induce lower innate immune response in mammalian cells by using Ψ-mRNAs.
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The INCOGNITO™ T7 mScript™ Ѱ-mRNA Production System provides all enzymes and enzyme-related reagents for making pseudouridine-containing, 5'-capped, 3'-polyadenylated mRNA. The kit includes modules for in vitro transcription of linear double-stranded DNA templates using the T7 mScript™ Enzyme Solution, the canonical nucleotides ATP, CTP, GTP and the modified nucleotide pseudouridine-5'-triphosphate, enzymatic capping of the RNA using the ScriptCap™ Cap 1 Capping System that contains both ScriptCap™ Capping Enzyme and 2'-O-Methyltransferase for making mRNA with a Cap 1 cap structure, A-Plus™ Poly(A) Polymerase for adding a 3'-poly(A) tail. and 5M NH4OAc as a convenient RNA/mRNA purification method. Kit includes a limited non-exclusive, non-transferable label license to use the products purchased from CELLSCRIPT™ solely for purchaser's own internal laboratory research use.
