ScriptCap™ 2′-O-Methyltransferase Kit
Prepare Cap 1-RNA from any source of Cap 0-RNA. Use simultaneously with ScriptCap™ m7G Capping System to produce virtually 100% Cap 1-capped RNA in a single reaction.
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The ScriptCap™ 2'-O-Methyltransferase Kit prepares Cap 1-RNA from any source of Cap 0-RNA. In part, Cap 1 methylation serves to increase the translation efficiency of the mRNA. ScriptCap™ 2'-O-Methyltransferase transfers a methyl group from the donor molecule SAM, S-adenosyl-methionine, to the 2'-O position of the penultimate nucleotide of a Cap 0-RNA to synthesize RNA with a Cap 1 structure. The Cap 0-RNA can be produced by enzymatically capping uncapped RNA using the CELLSCRIPT™ ScriptCap™ m7G Capping System or by in vitro transcription of a DNA template in the presence of a dinucleotide cap analog, for example, using the CELLSCRIPT™ MessageMAX™ T7 ARCA-Capped Message Transcription Kit V2. Cap 1-RNA can also be synthesized approaching 100% efficiency from uncapped RNA in a single reaction mixture that contains both the CELLSCRIPT™ ScriptCap™ m7G Capping System and ScriptCap™ 2'-O-Methyltransferase plus SAM. Product is for research use only (RUO).
