EZ-QC™ mRNA Poly(A) Tail Length Assay Kit
Quantitative mRNA poly(A) tail length using standard polyacrylamide gel electrophoresis (PAGE). Easy, cost-effective benchtop method for QC as an alternative to mass spec analysis. Assess tail lengths above the limits of HPLC and LC-MS based methods.
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The EZ-QC™ mRNA Poly(A) Tail Length Assay Kit provides quantitative mRNA poly(A) tail length analysis of synthesized mRNA. 3'-end polyadenylation, the addition of a poly(A) tail to mRNA, is a critical modification that influences mRNA stability and expression levels in eukaryotic cells, with longer tails generally providing increased stability and translation efficiency. 3'-poly(A) tails can be added co-transcriptionally (utilizing a template-encoded tail) or post-transcriptionally (via enzymatic addition) to in vitro transcribed (IVT) RNA as a step in the mRNA synthesis process. The EZ-QC™ mRNA Poly(A) Tail Length Assay Kit simplifies analysis and replaces tedious poly(A) tail length determination methods requiring reverse transcription, PCR or RNase H digests. The kit uses RNase A to digest non-adenine residues in the mRNA molecule, leaving the poly(A) tail intact to allow for length determination based upon standard polyacrylamide gel electrophoresis (PAGE) methodology. Product is for research use only (RUO).
