Single Cell CRISPR Guide Enrichment Kit
Amplify a CRISPR guide sequence derived from a CROP-style vector alongside your standard RNA Sequencing Library.
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The ScaleBio CRISPR Guide Enrichment Kit is modified from our standard ScaleBio Single Cell RNA Sequencing Kit, with the added benefit of capturing a CRISPR guide sequence derived from a CROP-style vector.
To maximize guide detection, we have included target-specific priming during reverse transcription, as well as PCR enrichment in the final step of library preparation.
This kit enables the processing of up to 500,000 cells or nuclei per run with extended throughput, as well as the multiplexing of up to 96 samples.
Cell Throughput
125,000 cells or nuclei per run, or up to 500,000 cells or nuclei per run with Extended Throughput Kit
Recommended input
10,000 cells per well (Initial Distribution)
Sample Throughput
Up to 96 samples per run
