PCR and Thermal Cycling Products & Reviews

PCR Master Mix(a,b) includes Nuclease-Free Water and PCR Master Mix, 2X. PCR Master Mix is a premixed, ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR. The PCR Master Mix has been optimized for use in routine PCR for amplifying DNA templates in the range of 0.2–2kb.

The GoTaq®Real-Time PCR Systems are ready-to-use 2X master mixes optimized for quantitative PCR in dye (using BRYT(R) Green Dye) or hydrolysis probe detection format.   The 1-Step and 2-Step RT-qPCR Master Mixes contain GoScript™ Reverse Transcriptase for efficient first-strand cDNA synthesis.     GoTaq Real-Time PCR Systems Features & Benefits: Higher fluorescence, often resulting in earlier Cqs Direct su…

The PowerPlex® Fusion System is a 24-locus multiplex for human identification applications including forensic analysis, relationship testing and research use.   The PowerPlex® Fusion System provides all of the materials needed for co-amplification and five-color fluorescent detection of 24 loci (23 STR loci and Amelogenin), including the CODIS core loci and the European Standard Set (ESS) loci. With 24 loci, the sys…

The GoTaq® Probe 1-Step RT-qPCR System is optimized for quantitative PCR assays in the hydrolysis probe detection format. The system enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® Probe qPCR Master Mix in single-step real-time amplification reactions. The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimi…

Process swabs and storage card punches for direct amplification.

Full-Length cDNA Synthesis for the Analysis of Rare or Long Messages

Optimized PCR master mix: Just add template, primers and water

Pfu DNA Polymerase is a high-fidelity, thermostable enzyme of approximately 90kDa isolated from Pyrococcus furiosus. The enzyme replicates DNA at 75°C, catalyzing the polymerization of nucleotides in the 5´→3´ direction in the presence of magnesium. Pfu DNA Polymerase also possesses 3´→5´ exonuclease (proofreading) activity. Base misincorporations that may occur during polymerization are rapidly excised by this proofreading ac…

GoTaq®2-Step RT-qPCR System is a reagent system for quantitative analysis of RNA using a two-step reverse transcription-quantitative PCR (RT-qPCR) method. The first step consists of cDNA synthesis using the GoScript™ Reverse Transcription System. Optimized reagents and ultra-active, GoScript™ Reverse Transcriptase result in high-efficiency, full-length cDNA synthesis from rare and abundant targets with up to 5 µg of RNA inp…

GoTaq® Hot Start Polymerase contains the high-performance GoTaq® DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The system is supplied with a tube of 25mM MgCl2, allowing optimization of the magnesium concentration in your reactions. It is also supplied with 5X Green GoTaq® Flexi Buffer and 5X Colorless GoTaq® Flexi Buffer. The buffers contain a compound that increases sample density, so tha…

GoTaq® Hot Start Master Mixes are premixed, ready-to-use solutions containing GoTaq® Hot Start Polymerase, magnesium, dNTPs and buffer. Reactions can be set up in less than a minute at room temperature; simply add your template, water and primers. Available with either green or colorless reaction buffers, which also serve as loading buffers, allowing you to go directly from thermal cycler to gel analysis. GoTaq® Hot Start Mast…

Original Eppendorf PCR tubes are manufactured to the highest Eppendorf quality standards. These thin-walled polypropylene tubes ensure efficient and homogeneous heat transfer in the sample thanks to the even wall thickness and smooth wall surface. Heat-sealed bags enclosed in boxes sealed with a PCR Clean sticker ensure the highest degree of purity. A frosted labeling area on the tube allows easy sample tracking

Product features Pink or light blue above 7°C, violet or dark blue when ice-cold DescriptionThe new Eppendorf PCR-Cooler maintains a sample temperature of 0°C*1 for over an hour and then changes color when the temperature has exceeded 7°C. Superior to an ice bath, PCR-Cooler is easy to handle, space-saving and free of contamination, which makes it the perfect preparation for your PCR reactions. The temperature of single tub…

Original Eppendorf PCR tubes are manufactured to the highest Eppendorf quality standards. These thin-walled polypropylene tubes ensure efficient and homogeneous heat transfer in the sample thanks to the even wall thickness and smooth wall surface. Heat-sealed bags enclosed in boxes sealed with a PCR Clean sticker ensure the highest degree of purity.

These automated pipetting systems are perfectly suited for PCR and qPCR setup, nucleic acid purification as well as low-throughput NGS library preparation yet they retain the flexibility for use as open platforms for diverse automated liquid handling applications. The epMotion 5073 series automates and simplifies traditionally complex, labor-intensive pipetting tasks, saving time and improving the reliability and reproducibili…

The DNA LoBind Tube finally provides the genomics user with the certainty that sample loss of genetic material caused by interaction with the plastic surface of the tube will be insignificant. With the combination of the purest selected types of polypropylene and specially developed production technologies, a tube has been developed that ensures nearly 100% recovery of DNA/RNA. A comparison of the recovery rates of various p…

The Mastercycler nexus X2 gives you the ability to run two totally independent protocols at the same time. Smaller assays fit nicely on the 32-well-block — larger assays can exceed 48 samples and run on the 64-well-block. The larger block is available with a gradient function. As every member of the Mastercycler nexus family, the Mastercycler nexus X2 can be combined with all other family members in a network of up to 3 units.…

The new Mastercycler® X50 elegantly combines speed, flexibility, and ease of use. With its intuitive touch screen offering precise fingertip control, the MC X50 PCR thermocycler enables easy PCR optimization in advanced molecular biology research and dependable standardization in routine PCR applications. It is also perfectly fitted for NGS.

Eppendorf real-time PCR Tube Strips feature an extremely thin wall for optimal heat transfer combined with high mechanical stability. In combination with their optical properties these are the ideal tube strips for any real-time PCR, especially for use with small reaction volumes. The Masterclear™ Cap Strips offer further advantages for your real-time PCR. The inverted dome of the Masterclear™ Cap Strips prevents scratching or…

Twist Bioscience, the leader in synthetic DNA with unparalleled precision at scale, is redefining targeted sequencing performance with the Twist Human Core Exome Kit. This kit includes library preparation and enrichment components, allowing the flexibility to lower sequencing costs, increase sample throughput, and achieve a higher depth of coverage across target regions with uncompromising quality.

Positive controls provide quality control measures for the verification and validation for point-of-care diagnostic tests including both next-generation sequencing (NGS) and reverse transcriptase-polymerase chain reaction (RT-PCR) assays. Twist offers fully synthetic SARS-CoV-2 RNA controls, available for dozens of distinct reference sequences.

96-well Real-Time PCR instrument with 10.2” touchscreen interface, 6 dye channel filters

96-well real-time PCR instrument for in-vitro diagnostic (IVD) use

The Streck ARM-D Kit, β-Lactamase is a multiplex real-time PCR kit for the rapid detection of β-lactam antibiotic resistance genes in Gram-negative bacteria. This comprehensive kit allows you to detect over 450 allelic variants in 9 clinically relevant carbapenemases, extended-spectrum beta-lactamases (ESBLs) and plasmid-mediated ampC gene families, KPC, NDM, OXA-48, IMP, VIM, DHA, CMY-2, CTX-M-14 and CTX-M-15. 

The Streck ARM-D Kit, OXA is a multiplex real-time PCR kit for the rapid detection of antibiotic resistance genes in Gram-negative bacteria. This comprehensive kit allows you to detect over 340 allelic variants within 6 OXA gene families, OXA-23, OXA-24/40, OXA-48, OXA-51, OXA-58 and OXA-143.