TaqMan SNP Genotyping Assays
With over 4.5 million SNP assays available, including 3.5 million HapMap SNPs, 70K cSNPs, and 160,000 validated assays, Applied Biosystems SNP Genotyping Assays make it easy to perform SNP genotyping studies with the precision of TaqMan® reagent-based chemistry. These assays provide SNP detection capabilities with high-confidence results. The large selection of SNP assays helps advance your screening, association, candidate…
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I am a great fan of TaqMan is a commonly used SNP genotyping method.
Nail microbiota identification
It is an advanced, mature, validated, and widely used technology. The TaqMan SNP genotyping assay is also called the "50-nuclease allelic discrimination assay". Each TaqMan genotyping assay contains two primers for amplifying the sequence of interest and two allele-specific and differently labeled TaqMan minor groove binder (MGB) probes for allele detection. Each allele-specific MGB probe is labeled with a fluorescent reporter dye (either a FAM or a VIC reporter molecule) in the 5' end and is attached with a fluorescence quencher to the 3' end. While the probe is intact, the proximity of the quencher dye to the reporter dye suppresses the reporter fluorescence. During the PCR amplification step, if the allele-specific probe is perfectly complementary to the SNP allele, it will hybridize to the target DNA segment and then get degraded by the 5'-nuclease activity of the Taq polymerase. The degradation of the probe results in the separation of the fluorophore from the quencher molecule, generating a detectable fluorescent signal. If a single point mismatch is present between the probe and the target DNA strand due to an SNP, the binding of the probe to DNA is destabilized during strand displacement in PCR, which will reduce the efficiency of probe cleavage and quenching of the fluorescent reporter dye.
Review Date: 23 May 2022 | Thermo Fisher Scientific
Zero optimization and faster, more accurate results.
SNP pre-designed assay
Easy to use TaqMan predesigned SNP genotyping assays quickly with zero optimization
Review Date: 19 Dec 2020 | Thermo Fisher Scientific
Fantastic service.
Exome SNP typing panel
Easy to use service with reliable and fast data generation. Will be using again.
Review Date: 1 Aug 2017 | Thermo Fisher Scientific
With over 4.5 million SNP assays available, including 3.5 million HapMap SNPs, 70K cSNPs, and 160,000 validated assays, Applied Biosystems SNP Genotyping Assays make it easy to perform SNP genotyping studies with the precision of TaqMan® reagent-based chemistry.
These assays provide SNP detection capabilities with high-confidence results. The large selection of SNP assays helps advance your screening, association, candidate region, candidate gene, or fine-mapping studies. Easy-to-use, single tube format. Robust assay design provides highly accurate, reproducible and reliable results. Easy assay ordering and a simple workflow provides quick results.
Choose from Human or Mouse pre-designed SNP Assays. All TaqMan® SNP Genotyping Assays are provided in a ready-to-use, single-tube format for the simplest genotyping workflow available and immediate results. Designed for use on all Applied Biosystems Real-Time PCR Systems and the TaqMan® OpenArray™ Genotyping System.
