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Q5® Site-Directed Mutagenesis Kit

Q5® Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours. The kit utilizes the robust Q5 Hot Start High-Fidelity DNA Polymerase along with custom mutagenic primers to create insertions, deletions and substitutions in a wide variety of plasmids. After PCR, the amplified material is added directly to a unique Kinase-Ligase-DpnI (KLD) enzyme mix for rapid, roo…

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New England Biolabs Inc.

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Average Rating 4.8

|4Scientists have reviewed this product

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Excellent kit, extremely versatile and easy to use. 5 stars

 

Average Rating 5.0

Application Area:

Molecular Biology

This product is terrific to use, and with the NEBasechanger online resource for primer design it's a breeze. I've used this kit many times to insert, delete, and substitute nucleotides for my cloning experiments and it's a joy every time. If only all kits could be this user-friendly!

Review Date: 9 Feb 2023 | New England Biolabs Inc.

Definitely useful for working with medium to large plasmids.

 

Average Rating 4.3

Application Area:

Coning contructs for knockdown in mammalian cell

Q5 Site-Directed Mutagenesis Kit is efficient and reliable. I canuse it for plasmids that are too large to amplify well by other high fidelity polymerases.

Review Date: 15 May 2021 | New England Biolabs Inc.

Best mutagenesis kit for all!

 

Average Rating 5.0

Application Area:

molecular biology

Great thing with this kit is: one kit for all. Insertion, point mutation, deletion can all be finished in here. The simplicity, efficiency, short work time are all good.

Review Date: 14 Mar 2019 | New England Biolabs Inc.

great kit for site directed mutagenesis

 

Average Rating 5.0

Application Area:

molecular cloning

Very easy to use with high-quality results. No limits on plasmid size and very versatile.

Review Date: 17 Apr 2018 | New England Biolabs Inc.

Q5® Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours.

The kit utilizes the robust Q5 Hot Start High-Fidelity DNA Polymerase along with custom mutagenic primers to create insertions, deletions and substitutions in a wide variety of plasmids. After PCR, the amplified material is added directly to a unique Kinase-Ligase-DpnI (KLD) enzyme mix for rapid, room temperature circularization and template removal. Transformation into high-efficiency NEB 5-alpha Competent E. coli, provided with the kit, ensures robust results with plasmids up to at least 14 kb in length.

Q5® Site-Directed Mutagenesis Kit Features:

  • Non-overlapping primer design ensures robust, exponential amplification, generating a high percentage of desired mutations from a wide range of templates
  • Intramolecular ligation and transformation into NEB high-efficiency competent cells results in high colony yield
  • Extremely low error rate of Q5 Hot Start High-Fidelity DNA Polymerase reduces screening time
  • Hot start polymerase enables room temperature reaction set-up
  • DpnI background reduction permits a wide range of starting template concentrations
  • Use of standard primers eliminates additional expenses from phosphorylated or purified oligos
  • Easy-to-use PCR master mix and unique multi-enzyme KLD mix offer convenience and quality
  • Rapid and direct treatment step proceeds at room temperature in 5 minutes

Q5® Site-Directed Mutagenesis Kit Applications:

  • Difficult PCR
  • Fast PCR
  • High-Throughput PCR
  • High-Yield PCR
  • Long Range PCR
  • Multiplex PCR
  • Routine PCR
  • Site Directed Mutagenesis

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