Q5® High-Fidelity DNA Polymerase
Q5® High-Fidelity DNA Polymerase is a high-fidelity, thermostable DNA polymerase with 3´→ 5´ exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. With an error rate > 100-fold lower than that of Taq DNA Polymerase and 12-fold lower than that of Pyrococcus furiosus (Pfu) DNA Polymerase, Q5 High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficul…
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Excellent polymerase, my go-to for everything PCR related
PCR
Works extremely well and is super reliable. I love this polymerase!
Review Date: 13 Feb 2023 | New England Biolabs Inc.
Super amplification without further PCR optimazation steps.
PCR with difficult DNA templates
We have finally found a perfect polymerase that works with the first PCR and does not need to be further optimized. We work a lot with plant DNA in the field of mangroves, macroalgae, seaweed. These templates in particular have many inhibitors (polyphenols and polysaccharides) and often make amplification difficult. NEB has a comparatively inexpensive alternative to the conventional, expensive polymerases.
Review Date: 22 Jun 2021 | New England Biolabs Inc.
Great and reliable results.
Molecular cloning
High quality product with flexibility in annealing temperature during PCR. Also can be relied on PCR products with no errors.
Review Date: 18 Jun 2021 | New England Biolabs Inc.
Great product has made my experiment go smooth with high quality results.
PCR for gene cloning
High flexibility with melting temperature, and good proof reading ability.
Review Date: 21 May 2021 | New England Biolabs Inc.
Great product!
DNA cloning
I always achieve high quality and reproducible results using this product.
Review Date: 17 Apr 2021 | New England Biolabs Inc.
Efficient PCR amplification for difficult templates.
PCR for recombinant DNA synthesis
The formulation that we purchased is the High Fidelity Hot Start Master Mix. Remarkably simple to use given the formulation without the need to pipette individual components. Accounting for the slightly higher annealing temperature needed for Q5, we were able to obtain several PCR products that were not amplified with our other favorite proofreading polymerase. It seemed to work particularly well with some of our genomic templates. Overall a great product.
Review Date: 17 Jun 2020 | New England Biolabs Inc.
Reliable!
Molecular biology
Reliable and fast. Price is not competitive.
Review Date: 26 Feb 2020 | New England Biolabs Inc.
Great Reagent
NGS amplicon library preparation
Great easy to use reliable Master mix. A proof reader with 3-5' exonuclease activity, great amplification of many targets but can permit binding and amplification to pseudogenes so careful with your designs.
Review Date: 1 Nov 2017 | New England Biolabs Inc.
Great!
Cloning, next gen library prep
The 2x mastermix makes any PCR set up super quick.
Review Date: 12 Jul 2016 | New England Biolabs Inc.
Q5® High-Fidelity DNA Polymerase is a high-fidelity, thermostable DNA polymerase with 3´→ 5´ exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification.
With an error rate > 100-fold lower than that of Taq DNA Polymerase and 12-fold lower than that of Pyrococcus furiosus (Pfu) DNA Polymerase, Q5 High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons.
Q5 High-Fidelity DNA Polymerase is supplied with an optimized buffer system that allows robust amplification regardless of GC content. The 5X Q5 Reaction Buffer contains 2 mM MgCl2 at final (1X) reaction concentrations and is recommended for most routine applications.
For GC-rich targets (≥ 65% GC), amplification can be improved by the addition of the 5X Q5 High GC Enhancer. Q5 High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes.
Q5® High-Fidelity DNA Polymerase Applications:
- High-fidelity PCR
- Cloning
- Long or Difficult Amplification
- High-throughput PCR
NEB Product Portfolio for Genomics, Proteomics and Cell Biology
This video presents New England Biolab’s product portfolio of restriction enzymes, recombinant polymerases, and modifying enzymes for a wide range of applications, from next generation sequencing to protein purification.
Important Tips for Working with Q5 DNA Polymerase
This video presents several important recommendations for working with Q5 DNA Polymerase from New England Biolabs that will help ensure successful DNA amplifications.
Q5® High-Fidelity DNA Polymerase from New England Biolabs
Watch this video to learn about Q5® High-Fidelity DNA Polymerase from New England Biolabs, offering speed, high-yield, and sensitivity, while also demonstrating robust performance on very high to very low GC targets.
New England Biolabs: How to Amplify GC Rich DNA?
Watch this video from New England Biolabs to learn how OneTaq and Q5 DNA polymerases optimize the amplification of AT rich and GC rich DNA targets respectively.
