miScript miRNA PCR Arrays
The miScript miRNA PCR Arrays accurately analyze the expression of multiple microRNA (miRNA) sequences at the same time on ANY real-time PCR instrument. This new generation of miRNA PCR Array builds on the original strengths of the RT² miRNA PCR Arrays* with new pathway content, expanded assay coverage plus added species all with the flexibility and sample preparation advantages of the miScript PCR System. The available 96-…
The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our Product Directory.
Product was used over last 8 years; were able to produce high yield of miRNA for various applications - miRNA Real-Time qRT-PCRs, pathway array RT-PCRs, gender determination RT-PCR; very efficient product, affordable, good quality reagents
Review Date: 12 Nov 2015 | QIAGEN
The miScript miRNA PCR Arrays accurately analyze the expression of multiple microRNA (miRNA) sequences at the same time on ANY real-time PCR instrument.
This new generation of miRNA PCR Array builds on the original strengths of the RT² miRNA PCR Arrays* with new pathway content, expanded assay coverage plus added species all with the flexibility and sample preparation advantages of the miScript PCR System. The available 96- or 384-well plates or Rotor Disc 100 arrays contain SYBR Green optimized and experimentally validated assays for either entire human, mouse, rat and dog miRNA genomes (miRNomes) or pathway-focused sets of miRNA assays.
Our proprietary miRNA detection technologies enable uniformly high PCR amplification efficiencies, allowing simultaneous detection of miRNA under uniform cycling conditions. These technologies also provide the miScript miRNA PCR Array with high sensitivity, specificity, and accuracy. miScript miRNA PCR Arrays are currently available for the human, mouse, rat and dog species, and individual miRNAs can be analyzed independently with miScript Primer Assays.
miRNA Biomarker Discovery – Overcoming Limiting Sample Material
Often the most valuable and informative samples — such as laser-captured samples, circulating tumor cells, or extracellular miRNA in body fluids — are the hardest to obtain in amounts sufficient for detailed miRNome profiling. This white paper presents an integrated, PCR-based system that reduces the amount of sample required for full miRNome profiling by several orders of magnitude and provides unparalleled reproducibility and precision. This advance enables detailed miRNA analysis on the smallest of samples and opens up new possibilities for biomarker development.
miRNA Profiling from Blood – Challenges and Recommendations
The discovery of stable miRNA species circulating in blood has led to increased research focus on disease-related variations in serum and plasma miRNA expression and the possibility that such variations could serve as noninvasive biomarkers for disease. Working with serum and plasma miRNA presents various challenges in purification and characterization. This paper outlines QIAGEN® recommendations for robust purification and quantification, as well as reliable data normalization and analysis.
Discover miRNA Biomarkers in Serum or Plasma Using Miscript® miRNA PCR Arrays
Changes in miRNA expression levels are indicative of a number of biological and disease states, and the stability of miRNAs in serum and plasma makes them attractive noninvasive biomarker candidates for cancer and other diseases. This application note describes use of the miRNeasy Serum/Plasma Kit in conjunction with the miScript PCR System in a pilot experiment to identify potential biomarkers for lung cancer.
Unlock miRNA Expression Profiles from FFPE Samples Using Miscript® miRNA PCR Arrays
miRNA expression profiling provides a valuable window into changes occurring in a biological system. Due to their stability and size, miRNAs can be readily extracted from FFPE samples using the miRNeasy FFPE Kit. This application note describes use of QIAGEN’s miScript PCR System to profile miRNA expression changes in total RNA samples isolated from FFPE normal and tumor lung tissues.
Webinar Highlights: Quantitative MicroRNA Expression Analysis in Tissues and Body Fluids
Whole genome profiling using small RNA sequencing, followed by qPCR based validation
Discover the Latest Epigenetics Methods and Technologies on SelectScience
New Application Notes, Product and Webinar
