Discover miRNA Biomarkers in Serum or Plasma Using Miscript® miRNA PCR Arrays
11 Dec 2013Changes in miRNA expression levels are indicative of a number of biological and disease states, and the stability of miRNAs in serum and plasma makes them attractive noninvasive biomarker candidates for cancer and other diseases. This application note describes use of the miRNeasy Serum/Plasma Kit in conjunction with the miScript PCR System in a pilot experiment to identify potential biomarkers for lung cancer.
Related Products
Request Quote for All Products
miScript miRNA PCR Arrays
QIAGENThe miScript miRNA PCR Arrays accurately analyze the expression of multiple microRNA (miRNA) sequences at the same time on ANY real-time PCR instrument. This new generation of miRNA PCR Array builds on the original strengths of the RT² miRNA PCR Arrays* with new pathway content, expanded assay coverage plus added species all with the flexibility and sample preparation advantages of the miScript PCR System. The available 96- or 384-well plates or Rotor Disc 100 arrays contain SYBR Green optimized and experimentally validated assays for either entire human, mouse, rat and dog miRNA genomes (miRNomes) or pathway-focused sets of miRNA assays. Our proprietary miRNA detection technologies enable uniformly high PCR amplification efficiencies, allowing simultaneous detection of miRNA under uniform cycling conditions. These technologies also provide the miScript miRNA PCR Array with high sensitivity, specificity, and accuracy. miScript miRNA PCR Arrays are currently available for the human, mouse, rat and dog species, and individual miRNAs can be analyzed independently with miScript Primer Assays.