Versatile, pH Stable (U)HPLC Columns: YMC-Triart Portfolio

Antibody-drug conjugates (ADCs) represent a rapidly evolving class of targeted therapies, with three distinct generations of conjugation methods. The first generation utilises lysine residues for drug attachment, while the second generation relies on cysteine coupling. The third generation, marks a significant advancement with site-specific conjugation, where cytotoxic drugs are attached at defined positions on the antibody, leading to a more homogeneous ADC with a precise drug-to-antibody ratio (DAR) of either 2 or 4. YMC Europe demonstrates the accurate DAR determination of a site-specific ADC using a YMC-Triart Bio C4 column, a robust wide-pore hybrid silica-based column designed to deliver reliable, high-resolution separations.

In this application note, YMC Europe introduces the YMC-Triart C18 UHPLC and MicroLC columns for proteomics analysis. The note emphasizes the necessity of highly sensitive methods in proteomics and native peptidomics analysis due to the wide range of concentrations at which peptides are present in biological samples. The note demonstrates the sensitivity improvement achieved by using UHPLC columns with different internal diameters and a micro-LC approach.

In response to the COVID-19 pandemic the anti-viral drug, molnupiravir, sold under the brand name Lagevrio®, was developed to treat mild to medium COVID-19 infection. Molnupiravir is a prodrug of the active substrate N4-hydroxycytidine (NHC), a synthetic ribonucleoside analogue. During the viral RNA replication the treatment introduces copying errors which inhibits the viral reproduction. In this application note from YMC Europe, explore how molnupiravir and its active agent β-d-N4-hydroxycytidine can be separated by using a YMC-Triart C18 column and a MS compatible mobile phase at pH 4.3 and relatively mild temperatures of 40°C.

In this application note, YMC Europe gives an overview, with experimental data, of its bioinert coated YMC-Accura Triart columns. The YMC-Accura Triart columns are ideal for highly sensitive LC/MS analyses and feature exceptional peak shapes with high sensitivities and excellent recoveries without column preconditioning. The YMC-Accura Triart columns are an alternative to the existing YMC-Triart metal-free, PEEK-lined columns. As the used column coating is less hydrophobic compared to the PEEK-lining, YMC-Accura columns are the ideal choice for more hydrophobic peptides which tend to show pronounced interactions with PEEK.

In LC analyses of sphingophospholipids, phosphate groups cause significant peak tailing, and therefore loss of sensitivity and reproducibility. In this application note, YMC discusses an improved method using YMC-Triart C18 metal-free columns.

Vitamin D3 (cholecalciferol) is essential for the human body. Low levels of vitamin D3 increase the risk of illness from a range of disorders. The serum level of vitamin D is monitored to exclude deficiency symptoms. As vitamin D products and nutritional supplements often contain vitamin D3 and/or vitamin D2 (ergocalciferol), usually the concentration of 25-hydroxy vitamin D3 (25-OH-D3, calcidiol) is determined. As vitamin D2, D3 and 25-OH-vitamin D3 are structurally similar substances, the (U)HPLC separation is therefore very challenging. Often, special columns in combination with complex eluent mixtures have to be used. This application note demonstrates the determination of vitamin D using YMC-Triart C18 ExRS.

The analysis of the original composition of plant material is necessary for such purposes as phenotype determination and quality control of medical cannabis used for therapeutic treatment. HPLC permits the determination of the original composition of plant cannabinoids by direct analysis. In this application note two fast methods are presented achieving high-performance in less than 9 min.

When carying out bio-separations, results may be optained with poor resolution. This may be due to interaction between analytes and column hardware. YMC presents YMC-Triart Metal-Free Columns as a solution to this problem. Metal free columns provide better peak shapes compared to regular columns and are therefore more suitable for high sensitivity analyses using LC/MS.

This application note displays the use of YMC Europe GmbH's YMC-Triart C18 (3 μm, 12 nm) 150 x 2.1 mm liquid chromatography column to analyze fumonisins.

Fatty acids have a well documented effect on health. On one hand unsaturated fatty acids are essential especially the omega-3 fatty acids which are beneficial in preventing heart diseases. The carbon-carbon double bonds of these essential fatty acids are cis type. On the other hand, fatty acids containing trans type double bonds are harmful to health. They are known to increase the risk of coronary heart diseases. During the industrial processing of foodstuffs such as margarine cis fatty acids are used. Therefore, it is essential to monitor the types of fatty acids in food. This application note demonstrates the determination of fatty acid isomers using YMC-Triart C18 ExRS.

New strategies pair high-performance chromatography with digital tools to tackle persistent pollutants

From sample limitations to isomeric separations, see how the right column choice transformed a challenging lipidomics project

Discover the latest technologies and methods advancing small molecule therapeutic discovery and development, from PROTAC quantification to microsampling innovations

Discover a range of new technologies and applications for biopharmaceutical development in this special editorial feature

Discover how changes in legislation have impacted on cannabis testing around the world, with solutions ranging from CPC to mass spectrometry

From laboratory food testing to operations out in the field, discover the latest strategies being used to monitor and secure the safety of the world's food