Robust bioinert columns for a new level of recovery: YMC Accura BioPro IEX columns
Bioinert coated YMC Accura BioPro IEX columns provide excellent peak shapes for sensitive compounds, ensuring high recovery from the very first injection.
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The non-porous hydrophilic polymer beads of the BioPro IEX stationary phases enable exceptionally high efficiencies and high throughput analyses. Even small sample variations can be easily separated and detected. The superior lot-to-lot reproducibility guarantees highly reliable results. BioPro IEX SF with its strong cation exchanger functionality is particularly ideal for analysing charge variants of monoclonal antibodies (mAbs) or other proteins. On the other hand, the strong anion exchanger BioPro IEX QF is the first choice for high-resolution analysis of oligonucleotides of different lengths as well as mAbs with lower pI.
Benefits from using YMC Accura BioPro IEX columns
YMC Accura columns feature a bioinert surface coating of the column body and frits. The combination of the bioinert coated column hardware and the high efficiency stationary phases of YMC Accura BioPro IEX columns provide excellent peak shapes for sensitive compounds. They ensure high recovery from the very first injection and at the same time prevent carry-over. This advantage stands out especially when analysing oligonucleotides compared to conventional PEEK hardware. YMC Accura BioPro IEX columns are not only very inert, but are also available with smaller column IDs, making them ideal for use in native IEX-MS analysis. Different column lengths are available for different purposes: Very short 30 mm columns provide high throughput, while 150 or 250 mm columns provide higher resolution. The new bioinert coated guard cartridges complete your application and provide optimum protection for your YMC Accura BioPro IEX column.
YMC Accura BioPro IEX Features:
- Superior resolution
- High recovery rate
- Excellent reproducibility
- High throughput
YMC Accura BioPro IEX Characteristics:
- Phases: non-porous strong anion exchanger (QF), non-porous strong cation exchanger (SF)
- Particle size: 3, 5µm
- pH range: 2 – 12
- Max. temperature: up to 80 °C
- Hardware: bioinert coated stainless-steel hardware
- Internal diameter: 4.6 and 2.1 mm (ideal for LC-MS)
- Length: 30-250 mm
- Bioinert guard cartridges available: 5 mm x 2.1 or 4.6 mm ID
High recoveries from IEX analysis of mAbs
Ion exchange chromatography (IEX) is commonly used to characterize charge-sensitive biomolecules like monoclonal antibodies (mAbs). While PEEK is typically used for column hardware, bioinert coated stainless steel offers an alternative with different surface properties, being less hydrophobic. YMC Europe highlights this distinction through the analysis of bevacizumab, where both a standard PEEK column and a bioinert coated YMC Accura BioPro IEX SF column were tested. Consecutive injections of bevacizumab showed that preconditioning is generally necessary for complete recovery on both columns.
Bioinert coated hardware in oligonucleotide analysis
Oligonucleotides can modulate gene expression, offering potential treatments for various diseases. The rapidly growing field of therapeutic oligonucleotides requires robust analytical methods for quality and safety. Anion exchange chromatography (AEX) is suitable but often results in poor peak shape and low recovery due to oligonucleotide adsorption to column hardware. YMC Europe presents YMC Accura BioPro IEX QF columns, with bioinert coated hardware, enabling reliable and reproducible analyses. The BioPro IEX columns are based on porous and non-porous hydrophilic polymer beads with low nonspecific adsorption. YMC reports that they also show higher binding capacity and higher recovery of biomolecules compared to conventional ion exchange columns.
