Size Exclusion Chromatography (SEC) Column for mAb Separations
YMC-SEC MAB is a silica-based size exclusion chromatography column utilizing a dihydroxypropyl stationary phase bonded to a silica gel base, specifically designed for antibody separations.
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YMC-SEC MAB is a silica-based size exclusion chromatography column utilizing a dihydroxypropyl stationary phase bonded to a silica gel base, specifically designed for antibody separations. This material offers a 250Å pore size in 3µm particle size, making it an excellent choice for separating aggregates from monomer, as well as effectively resolving lower molecular weight fragments. YMC-SEC MAB exhibits excellent resolution and peak shape over a wide molecular weight range of 10kDa to 700kDa.
Features & Benefits:
- Silica-based SEC column specifically designed for monoclonal antibodies
- Optimized for separating aggregates and fragments of mAb’s
- High resolution and excellent peak shapes
- Available in 3µm particle size with 250Å pore
- Molecular weight range of 10kDa to 700kDa
- Suitable for SEC analysis of antibody drug conjugates using organic-containing mobile phases
DAR determination using third generation conjugation method
Antibody-drug conjugates (ADCs) represent a rapidly evolving class of targeted therapies, with three distinct generations of conjugation methods. The first generation utilises lysine residues for drug attachment, while the second generation relies on cysteine coupling. The third generation, marks a significant advancement with site-specific conjugation, where cytotoxic drugs are attached at defined positions on the antibody, leading to a more homogeneous ADC with a precise drug-to-antibody ratio (DAR) of either 2 or 4. YMC Europe demonstrates the accurate DAR determination of a site-specific ADC using a YMC-Triart Bio C4 column, a robust wide-pore hybrid silica-based column designed to deliver reliable, high-resolution separations.
Analyzing Bevacizumab, its aggregates and fragments
Due to the sizes of monoclonal antibodies (MAbs, about 150 kDa), size exclusion chromatography (SEC) is a standard technique for analysing MAbs such as Bevacizumab (Avastin®). In this application note, YMC’s dedicated SEC column for antibodies, YMC-SEC MAB, is used to separate the monomer antibody from its aggregates as well as from degradation products in a single run.
SEC Analysis of MAb Fragments with Increased Throughput and High Resolution
In this application note, YMC outlines how the YMC-Pack Diol-200 SEC and YMC-SEC MAB columns promise fast analysis of antibody fragments and degradation products at a high resolution.
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