Member since: 2021
Best tested RP column for mAb fragments separation
Application Area: Separation of mAb fragments
"Column resolution is excellent. After conditioning with 0.5%TFA the column can be used without TFA in the mobile phase thus improving MS ionisation. Use a shallow gradient ranging from 20 to 40% organic over a wide run time 30min to achieve ideal separation of mAb fragments."
Use the new Thermo Scientific™ MAbPac™ RP columns for high-performance reversed-phase (RP) HPLC and LC/MS characterization of monoclonal antibodies (mAbs), fragments, variants, antibody drug conjugates (ADCs), PEGylated proteins, and bispecific proteins. These columns have excellent resolution, long column lifetime, and low carry-over. The surface chemistry, particle size, and pore size, have been optimized to for high resolution separations.
Compatible with organic solvent used in HPLC and MS under RP conditions
Efficient with low carry over
Designed for reversed-phase biomolecule LC and LC/MS separations