Thermo Scientific™ MAbPac™ RP LC Columns
Use the new Thermo Scientific™ MAbPac™ RP columns for high-performance reversed-phase (RP) HPLC and LC/MS characterization of monoclonal antibodies (mAbs), fragments, variants, antibody drug conjugates (ADCs), PEGylated proteins, and bispecific proteins. These columns have excellent resolution, long column lifetime, and low carry-over. The surface chemistry, particle size, and pore size, have been optimized to for high resolut…
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Best tested RP column for mAb fragments separation
Separation of mAb fragments
Column resolution is excellent. After conditioning with 0.5%TFA the column can be used without TFA in the mobile phase thus improving MS ionisation. Use a shallow gradient ranging from 20 to 40% organic over a wide run time 30min to achieve ideal separation of mAb fragments.
Review Date: 10 Nov 2021 | Thermo Fisher Scientific
Use the new Thermo Scientific™ MAbPac™ RP columns for high-performance reversed-phase (RP) HPLC and LC/MS characterization of monoclonal antibodies (mAbs), fragments, variants, antibody drug conjugates (ADCs), PEGylated proteins, and bispecific proteins. These columns have excellent resolution, long column lifetime, and low carry-over. The surface chemistry, particle size, and pore size, have been optimized to for high resolution separations.
Compatible with organic solvent used in HPLC and MS under RP conditions
- Resin is based on a hydrophobic supermacroporous 4µm polymer particles
- Stable at extreme pH (0 to 14) and high temperature (up to 110 °C)
Efficient with low carry over
- 1,500 Å large pore size polymeric resin;
- Efficient separation of protein molecules with very low carry over
Designed for reversed-phase biomolecule LC and LC/MS separations
- Superior resolution power for monoclonal antibodies and related substances
- High efficiency with low carry-over
- Excellent MS compatibility
- Wide operating pH range: 0 to 14
- High temperature stability: up to 110 °C
- High throughput
Top-down Characterization of Monoclonal Antibody on an Orbitrap Fusion Lumos™ Tribrid Mass Spectrometer
This application note describes a method for mass-analysis and top-down characterisation of a monoclonal antibody, conducted on a modified Orbitrap Lumos mass spectrophotometer. LC-MS analysis was performed on the intact monoclonal antibody, the IdeS sub-units and the reduced heavy chain product. ETD HD, UVPD and CID top-down fragmentation modes were applied to obtain the optimal sequence coverage.
