Rapid DNA Dephos & Ligation Kit by Roche Applied Science - a member of the Roche Group

Upgrade from the Rapid DNA Ligation Kit and choose the new Rapid DNA Dephos & Ligation Kit for fast and efficient dephosphorylation and ligation of sticky- or blunt-end DNA fragments. The convenient kit enables ligation of DNA in 5 minutes and dephosphorylation in 10 minutes. This kit contains the new recombinant rAPid Alkaline Phosphatase, the tool of choice for dephosphorylation of 5' phosphates from nucleic acids. This kit minimizes the time needed to perform diverse applications, includi... Read more
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Rapid DNA Dephos & Ligation Kit by Roche Applied Science - a member of the Roche Group product image
Rapid DNA Dephos & Ligation Kit

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Upgrade from the Rapid DNA Ligation Kit and choose the new Rapid DNA Dephos & Ligation Kit for fast and efficient dephosphorylation and ligation of sticky- or blunt-end DNA fragments. The convenient kit enables ligation of DNA in 5 minutes and dephosphorylation in 10 minutes. This kit contains the new recombinant rAPid Alkaline Phosphatase, the tool of choice for dephosphorylation of 5' phosphates from nucleic acids.

This kit minimizes the time needed to perform diverse applications, including

  • cloning of fragments into either plasmid or phage vectors
  • linker ligation
  • recircularization of linear vector DNA
  • generation of libraries

    Note: To purify ligated DNA for electroporation, use the High Pure PCR Product Purification Kit.

Benefits

  • Save time with a convenient, easy-to-use kit: Phosphate groups are removed in 10 minutes and DNA is ligated in 5 minutes.
  • Order one product to obtain a complete solution: All reagents needed for dephosphorylation and ligation are supplied in one convenient package.
  • Simplify your research with easy protocols: Ligation reaction is performed at room temperature and dephosphorylation can be performed directly in restriction enyzme digests.
  • Reduce background: This optimized kit enables highly efficient ligation reactions.

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