Mouse Serpin E1/PAI-1 DuoSet ELISA by Bio-Techne

Manufacturer Bio-Techne  |  Available Worldwide
4.0
/
5.0
  |  1 reviews
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse Serpin E1 / PAI-1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.


Mouse Serpin E1/PAI-1 DuoSet ELISA by Bio-Techne product image
Mouse Serpin E1/PAI-1 DuoSet ELISA

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Average Rating: 4.0
1 Scientist has reviewed this product

4 out of 5
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money


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  • Organization: University of Michigan



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Experiment worked in first trial!
Rating: 4.0

  • Application Area: running ELISA on mouse blood plasma

"This was my first ELISA experiment. I got the result that was expected. This kit is supplied with all the good reagent and protocol. I tried to stick to the recommended protocol."

Review date: 18 Dec 2018 | Mouse Serpin E1/PAI-1 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse Serpin E1 / PAI-1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

  • Format: 96-well strip plate
  • Sample volume required: 100 µL
  • Sufficient materials for five 96-well plates

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

 

  • PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
  • Wash Buffer: (Catalog # WA126), or equivalent
  • Reagent Diluent*
  • Blocking Buffer*
  • Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
  • Stop Solution: 2 N H2SO4 (Catalog # DY994)
  • Microplates: R&D Systems (Catalog # DY990), or equivalent
  • Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .