Molecular Beacons by LGC, Biosearch Technologies

4.0
/
5.0
  |  1 reviews
Molecular Beacons are a type dual-labeled FRET probes incorporating a quencher and a fluorophore reporter molecule. They differ from traditional dual-labeled probes, due to the incorporation of a short (~5 b.p.) complementary stem sequence at the 3' and 5' ends.


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Molecular Beacons
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Average Rating: 4.0
1 Scientist has reviewed this product

4 out of 5
Ease of use
4 out of 5
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4 out of 5
Value for money


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  • Member since: 2017

  • Organization: VARI



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Rating: 4.0

  • Application Area: Biomarker, FACS

"I am very pleased with the product yield and purity. I usually receive the custom made product within two weeks after I ordered it, which is great."

Review date: 31 Jul 2017 | Molecular Beacons

Molecular Beacons are a type dual-labeled FRET probes incorporating a quencher and a fluorophore reporter molecule. They differ from traditional dual-labeled probes, due to the incorporation of a short (~5 b.p.) complementary stem sequence at the 3' and 5' ends.

These complementary sequences hybridize to form a “stem-loop” structure which holds the reporter and quencher close together in space. This alternate conformation makes Molecular Beacons more discriminatory, allowing them to resolve different target sequences differing by a single nucleotide. Molecular Beacons also differ from traditional dual-labeled probes because they do not rely on probe hydrolysis from the 5' exonuclease activity of Taq polymerase to generate its fluorescence, therefore, a post-PCR melt curve is possible.

Just like standard Dual-labeled BHQ® probes it is possible to multiplex assays using Molecular Beacons. Multiplexing allows for the detection of several targets simultaneously using multiple spectrally resolved fluorescent probes.

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Molecular Beacons by LGC, Biosearch Technologies product image

Molecular Beacons

Manufacturer LGC, Biosearch Technologies

4.0 / 5.0 | 1 reviews