Use the iScript Explore One-Step RT and PreAmp Kit to generate preamplified cDNA directly from RNA in a single step. This kit is well suited for detecting many targets from a limited sample or for reliable detection of targets expressed at low levels. The patented Sso7d fusion protein provides unbiased target specific preamplification of up to 100 targets. The kit also integrates an effective genomic DNA removal digestion step, helping ensure only the transcripts of interest are detected by qPCR.
Simply create gDNA-free, preamplified cDNA by combining the reagents in this kit with RNA sample and PreAmp Assays. This kit contains all of the reagents needed to easily create an unbiased 1,000-fold amplification of cDNA.
Preamplification is a powerful tool to enable highly sensitive detection of a large number of targets from a limited sample. The combination of proprietary buffers and the Sso7d fusion technology enables the iScript Explore One-Step RT and PreAmp Kit to generate unbiased preamplification reactions regardless of the amount of target present.
Contaminating gDNA in RNA samples can be amplified in RT-qPCR experiments that use exonic primers, biasing the results for the transcript of interest. This problem is especially acute for low expressing genes, such as lncRNAs — even a few copies of contaminating gDNA can shift Cq values dramatically. The iScript Explore One-Step RT and PreAmp Kit addresses the issue with a specially formulated DNase that effectively eliminates gDNA from purified RNA.
Choose the preamplification product that best suits your needs.