IP-One Assay Kit by Cisbio Bioassays

Manufacturer Cisbio Bioassays
A new secondary messenger for Gq coupled receptor investigation.Cisbio’s IP-One assay, based on its proprietary HTRF® technology, is the first HTS immunoassay that can easily detect inositol(1)phosphate (IP1), a stable second messenger which tightly correlates with Gq-coupled receptor activity. This assay displays all the characteristic advantages of an HTRF® system: totally homogeneous, detection via fluorescence, signal stability, miniaturization down to uHTS levels (less than 10 µl), "mix & m... Read more


IP-One Assay Kit by Cisbio Bioassays product image
IP-One Assay Kit

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A new secondary messenger for Gq coupled receptor investigation.

Cisbio’s IP-One assay, based on its proprietary HTRF® technology, is the first HTS immunoassay that can easily detect inositol(1)phosphate (IP1), a stable second messenger which tightly correlates with Gq-coupled receptor activity.

This assay displays all the characteristic advantages of an HTRF® system: totally homogeneous, detection via fluorescence, signal stability, miniaturization down to uHTS levels (less than 10 µl), "mix & measure" type protocols limited to just a few dispensing steps, and use in "single plate" mode on cultured cells.

IP-One has been validated in a wide variety of different situations: stable lines, transitory transfections, different cell types (CHO, HEK, 1321N1). The validations also conclusively demonstrated the assay’s performances in the presence of cell lines using Ga16 or Gqi9 type chimeric constructions.

With IP-One and cAMP, Cisbio offers a reliable and functional platform for investigating and screening GPCR activity.