Invitrogen™ LIVE/DEAD™ Fixable Near-IR Dead Cell Stain Kit by Thermo Fisher Scientific

The LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit provides the stable components to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. The amine-reactive LIVE/DEAD Fixable Near-IR Dead Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines in cells with compromised membranes, whereas this reactivity is restricted to amines on the cell surface in viable cells. The fluorescence difference between dead and live cells is greater than 50-fold. The staining pattern is preserved following formaldehyde fixation.

Features

• Stable—dyes are freeze dried in separate vials to maintain stability
• Robust—staining pattern is the same before and after fixation
• Bright signal—allows for easy distinction between live/dead cells in single channel

The LIVE/DEAD Fixable Near-IR Stain can be excited using either a red laser. The near-IR fluorescent dye has an excitation maximum of approximately 633 nm that is excited well with a red or HeNe laser, and an emission of approximately 750 nm. Since live and dead cells can be discriminated using a single dye and a single channel of a flow cytometer, it is an ideal choice for multi-color experiments.