Human TNF-alpha/TNFSF1A Quantikine ELISA Kit by Bio-Techne

Manufacturer Bio-Techne
3.9
/
5.0
  |  2 reviews
R&D Human TNF-alpha/TNFSF1A Quantikine ELISA Kit - Human TNF-alpha Immunoassay for the quantitative determination of human tumor necrosis factor alpha (TNF-alpha) concentrations in cell culture supernates, serum, and plasma. The R&D Quantikine Human TNF-alpha Immunoassay is a 3.5 - 4.5 hour solid phase ELISA. This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for TNF-alpha has been pre-coated onto a microplate. R&D Systems Qua... Read more


Human TNF-alpha/TNFSF1A Quantikine ELISA Kit by Bio-Techne product image
Human TNF-alpha/TNFSF1A Quantikine ELISA Kit

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Average Rating: 3.9
2 Scientists have reviewed this product

4 out of 5
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money


  • Status:

    Advanced Reviewer
  • Member since: 2009

  • Organization: Laboratorios Scantibodies S.A. de C.V.



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    3 out of 5
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The test offers consistency in results.
Rating: 3.7

  • Application Area: Analyze serum protein concentrations

"The product is of good quality and consistency, but it would be good if incubation times would be shorter."

Review date: 15 Jan 2016 | Human TNF-alpha/TNFSF1A Quantikine ELISA Kit
  • Status:

    Expert Reviewer

  • Member since: 2002

  • Organization: University of Utah- Vascular Lab



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Rating: 4.0

  • Application Area:ELISA Kits

"The instructions are very clear, and the kit has all the reagents needed to run the assay. The calculations to determine the sample concentrations were a little bit tricky for me, but once I figured it out it was not a problem. The storage recommendations are very important; don't save prepared chemicals longer than recommended or under storage conditions not recommended. The standards curve came out looking really good except for a few times when I neglected to notice the recommended storage conditions for the TNF-alpha stock solution. "

Review date: 24 Jun 2014 | Human TNF-alpha/TNFSF1A Quantikine ELISA Kit
R&D Human TNF-alpha/TNFSF1A Quantikine ELISA Kit - Human TNF-alpha Immunoassay for the quantitative determination of human tumor necrosis factor alpha (TNF-alpha) concentrations in cell culture supernates, serum, and plasma.

The R&D Quantikine Human TNF-alpha Immunoassay is a 3.5 - 4.5 hour solid phase ELISA. This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for TNF-alpha has been pre-coated onto a microplate.

R&D Systems Quantikine ELISA Kits are complete and ready-to-use.

Features of the R&D Quantikine ELISA Kits include:

  • Complete ready to use kits 
  • Exhaustively tested for superior quality and reproducibility 
  • Detailed protocol booklets include inter- and intra-assay precision, recovery, linearity, sensitivity, and sample value data 
  • Colorimetric detection

R&D Quantikine ELISA Kit Contents:

Kit contents may vary slightly.

Pre-coated 96-well Microplate 

  • Conjugated Detection Antibody 
  • Calibrated Immunoassay Standard 
  • Assay Diluent 
  • Calibrator Diluent(s) 
  • Wash Buffer 
  • Color Reagent A and B 
  • Stop Solution 
  • Plate Sealers

Product Overview

Human TNF-alpha/TNFSF1A Quantikine ELISA Kit by Bio-Techne product image

Human TNF-alpha/TNFSF1A Quantikine ELISA Kit

Manufacturer Bio-Techne

3.9 / 5.0 | 2 reviews