Average Rating: 5.0
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Simple and fast protein purification!
"The product enables simple and fast His-Tag purification for small volumes. For the purification of proteins produced within our cell-free expression system the kit is a valuable tool."Review date:
11 Dec 2019 | His-Spin™ Protein Miniprep Kit
"Product is very easy to use, purification is done quickly, protein was very pure and active afterwards."Review date:
05 Jun 2019 | His-Spin™ Protein Miniprep Kit
The His-Spin Protein Miniprep provides researchers with a method for fast His-tagged protein purification. The easy-to-follow procedure is based on a nickel-charged His-Affinity Gel (IMAC), innovative protein purification, and unique Zymo-Spin Column technology. Up to 1 mg of His-tagged protein can be purified in as little as 5 minutes and can be eluted into as little as 100 µl of the provided His-Elution Buffer. The purified protein can be used directly for enzymatic assays, protein biochemical analyses, SDS PAGE, as well as other protein based applications. The His-Spin Protein Miniprep has been optimized to yield maximal protein purity indices: a single protein band is often visualized following Coomassie Blue staining of proteins in SDS-PAGE gel (see figure below). The straightforward spin-wash-elute protocol dramatically simplifies protein purification and results are obtained in minutes, not hours!