Member since: 2021
Organization: University of Amsterdam
Application Area: PCR clean-up
"The product works amazingly well. Easy to use, but quite expensive for "normal" PCR purifications. However, I strongly recommend this product for library prep with little DNA."
The HighPrep™ PCR Clean-up System is a paramagnetic beads-based post PCR clean-up reagent designed for efficient purification of DNA fragments and tight size-specific selection of amplicons. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic bead particles. Highly purified DNA is eluted with low salt elution buffer or water which can be used directly for downstream applications, such as library construction, next-generation sequencing (NGS), Sanger sequencing, cloning, restriction digestions, adapoter ligation, microarrays, and so on. This protocol can be used for manual procedure as well as as a guideline for adapting the kit to automatic liquid handling instruments. The HighPrep™ PCR Clean-up Reagent is perfectly suited for amplicon size selection, and NGS library preparation for sequencing as well as for post PCR clean-up of shorter amplicons or restriction enzyme digestion products.