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FirePlex® ​​Multiplex miRNA assays

Multiplex miRNA assays with FirePlex particle technology enables simultaneous profiling of 65 miRNAs directly from small amounts of biofluid or FFPE, without RNA purification or pre-amplification, with assay readout on a standard flow cytometer

Abcam plc

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Application Area:

RNA quantitation

Abcam FirePlex multiplex assay service provides results for miRNA profiles associated with different diseases. miRNA Panel – Cardiology V2 is very effective and gives accurate results. Fast and complete service from the team.

Review Date: 2 Feb 2021 | Abcam plc

Multiplex miRNA assays with FirePlex particle technology enables simultaneous profiling of 65 miRNAs directly from small amounts of biofluid or FFPE, without RNA purification or pre-amplification, with assay readout on a standard flow cytometer

Small sample input

  • 20ul of serum, plasma, urine, exosomes, single 5 µm FFPE section.

Extraction-free

  • Eliminate extraction bias, reduce variability, simplify workflow, and conserve your sample.

Easy and rapid data analysis with FirePlex Analysis Workbench software

  • Carry out complex analysis and visualization, as well as export publication-quality heat maps and graphs in under ten minutes.

No specialized equipment is required

  • Readout is performed on standard flow cytometers

Parallel processing of 96 samples

  • Multiplexed detection of up to 70 targets (65 miRNA and 5 internal controls) in 96-well plate

A customizable biomarker development platform

  • Select between pre-designed panels for key research areas, larger discovery panels, or design custom panels to detect any miRNA from any species, whether annotated in miRBase or not.

Multiplex miRNA assays

  • FirePlex® miRNA Assay V2 - Rodent Discovery Panels (ab217053)
  • FirePlex® miRNA Assay V2 - Human Discovery Panels (ab217049)
  • FirePlex® miRNA Panel – Neurology V2 (ab218371)
  • FirePlex® miRNA Panel – Liver Tox V2 (ab218370)
  • FirePlex® miRNA Panel – Immunology V2 (ab218369)
  • FirePlex® miRNA Panel – Cardiology V2 (ab218368)
  • FirePlex miRNA Panel - Pancreatic Toxicity (ab219510)
  • FirePlex miRNA Panel - Cardiac Toxicity (ab219509)
  • FirePlex miRNA Panel - Kidney Toxicity (ab219508)
Application eBookLife Sciences

Master multiplex assay design for drug discovery

For high-throughput screening to be an effective tool for identifying promising candidates in drug discovery, it requires scalable, high-quality immunoassays to identify the candidates quickly, efficiently, and with minimal costs.

In this free application guide, find out how multiplexing can address those challenges by enabling you to measure multiple target analytes in a single reaction, exponentially increasing the number of data points collected whilst reducing workflow and sample volumes. Plus, discover the key considerations when choosing a multiplex assay and a comprehensive overview of the various multiplex assay techniques used in screening.


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