Member since: 2022
Organization: Cornell University
First reported generation cat iPSCs using sendai viruses
Application Area: Reprogramming feline fibroblasts
"I am reprogramming feline fetal fibroblasts using sendai virus reprogramming kit. So far, no one has generated iPSCs from feline cells using Sendai viruses may be due to a lack of knowledge about reprogramming cocktails and appropriate culture conditions. I have used the above product to reprogram cat fetal fibroblasts and so have achieved promising results. The product is super easy to handle, even for non-exerts on stem cell biology. The product guide, even more, simplifies the usage. Highly recommended."
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The CytoTune™-iPS 2.0 Sendai Reprogramming Kit improves upon the well-referenced CytoTune™-iPS Sendai Reprogramming Kit. Improvements in the 2.0 system include increased efficiency, lower cytotoxicity, and faster clearance in the generation of induced pluripotent stem cells. This system continues to be easy to use and works with many different somatic cell types, using Sendai particles to deliver Yamanaka factors, which have been shown to be critical for efficient generation of iPSCs. This kit includes three vector preparations: polycistronic Klf4–Oct3/4–Sox2, cMyc, and Klf4.